有氧运动对2型糖尿病大鼠肾组织STAT3及VEGF表达的影响

Effects of Aerobic Exercise on the Expression of STAT3 and VEGF in Kidney Tissue of Type 2 Diabetes Rats

目的:研究有氧运动对2型糖尿病大鼠肾组织转录激活因子3(activators of transcription 3, STAT3)及血管内皮生长因子(vascular endothelial growth factor, VEGF)表达的影响及其机制。方法:健康雄性SD大鼠55只,采用高糖高脂饲料喂养加腹腔注射低剂量链脲佐菌素(STZ)制作2型糖尿病大鼠模型。实验分为正常对照组,糖尿病安静组和糖尿病运动组。糖尿病运动组大鼠进行8周的有氧运动,其它两组不进行干预。检测血糖、24 h UA、BUN、Scr的变化;检测肾组织VEGF、STAT3和STAT3 m RNA的表达。结果:与正常对照组相比,糖尿病安静组大鼠血糖、24 h UA、BUN和Scr均显著升高(P<0.05);糖尿病运动组大鼠血糖、24 h UA、BUN和Scr均较糖尿病安静组显著下降(P<0.05)。糖尿病安静组大鼠肾组织VEGF、STAT3和STAT3 m RNA的表达量分别为(0.417±0.121)、(0.527±0.139)和(2.86±0.32),均较正常对照组明显增加(P<0.05);糖尿病运动组大鼠肾组织VEGF、STAT3和STAT3 m RNA的表达量分别为(0.225±0.076)、(0.298±0.082)和(1.74±0.25),均较糖尿病安静组明显减少(P<0.05)。结论:有氧运动对糖尿病所致肾损伤有一定改善作用,其保护机制可能与下调肾组织STAT3 m RNA的表达,使STAT3和VEGF合成减少有关。

Objective：To investigate the effect of aerobic exercise on STAT3 and VEGF in kidney tissue of type 2 diabetes rats and its mechanism.Methods：The model of type 2 diabetic rats was established through 55 SD rats fed high-fat diet together with intraperitoneal infecting after a low dose of STZ.The rats were randomly divided into normal control group,diabetic control group and diabetic exercise group.The diabetic exercise group was intervened of exercise for 8 weeks.We examined blood glucose concentrations,24 h UA,BUN,Scr,and the expressions of VEGF,STAT3 and STAT3 mRNA in kidney tissue.Results：Compared with normal control group,the blood glucose concentrations,24 h UA,BUN and Scr increased significantly in diabetic control group（P〈0.05）.The blood glucose concentrations,24 h UA,BUN and Scr decreased significantly in diabetic exercise group than in diabetic control group（P〈0.05）.The expressions of VEGF,STAT3 and STAT3 mRNA in diabetic control group were（0.417±0.121）,（0.527±0.139） and（2.86±0.32）respectively,and they were significantly higher than normal control group（P〈0.05）;The expressions of VEGF,STAT3 and STAT3 mRNA in diabetic exercise group were（0.225±0.076）,（0.298±0.082） and（1.74±0.25） respectively,and they were significantly lower than diabetic control group（P〈0.05）.Conclusion：Aerobic exercise could protect renal injury in type 2 diabetes rats,which may be related to downregulate the expression of STAT3 mRNA,and decrease the synthesis of STAT3 and VEGF.