Beclin1与p53在唾液腺多形性腺瘤和癌在唾液腺多形性腺瘤中的表达及与临床病理因素的关系研究

Expressions of Beclin1 and p53 in Pleomorphic Adenoma, Carcinoma in Pleomorphic Adenoma and its Relationship with Clinicopathological Factors

目的:探讨自噬体相关基因Beclin1与p53基因在唾液腺多形性腺瘤(PA)和癌在唾液腺多形性腺瘤(CPA)中的表达及其与临床病理因素的关系。方法:选择2013年1月至2017年8月于我院手术切除的PA标本45例作为PA组,CPA标本32例作为CPA组,正常腮腺组织30例作为对照组,应用免疫组化SP法对三组Belin1与p53基因表达情况进行检测,分析CPA组Beclin1、p53表达情况与临床病理因素关系及Beclin1、p53表达的相关性。结果:三组Beclin1、p53阳性表达率存在统计学差异,CPA组Beclin1阳性表达率低于PA组和对照组,CPA组和PA组p53阳性表达率高于对照组,且CPA组高于PA组(P<0.05)。CPA组Beclin1、p53表达情况与TNM分期、淋巴结转移有关,TNM分期为III-IV期患者Beclin1阳性表达率低于I-II期患者,p53阳性表达率高于I-II期患者,淋巴结转移患者Beclin1阳性表达率低于无淋巴结转移患者,p53阳性表达率高于无淋巴结转移患者,差异均有统计学意义(P<0.05),CPA组Beclin1、p53表达情况与年龄、性别、肿瘤直径、侵袭性无关(P>0.05)。经Spearman相关性分析可得,CPA患者Beclin1和p53的表达呈负相关关系(r=-0.839,P=0.000)。结论:PA及CPA中存在Beclin1异常低表达,p53异常高表达,CPA患者Beclin1、p53表达情况与TNM分期、淋巴结转移有关,且两者表达呈负相关。

Objective： To analyze the expressions of autophagic related genes Belin1 and p53 in pleomorphic adenoma（PA）, carcinoma in pleomorphic adenoma（CPA） and its relationship with clinicopathological factors. Methods： 45 cases of PA excised in our hospital from January 2013 to August 2017 were selected as PA group, 32 cases of CPA specimens as CPA group, 30 normal parotid tissue as control group, the expression of Belin1 and p53 gene in three groups were detected by immunohistochemical SP method, the relationship between the expression of Beclin1 and p53 in the CPA group and the clinicopathological factors, the correlation of the expression of Beclin1 and p53 were analyzed. Results： There were statistical differences in the positive rates of Beclin1 and p53 in the three groups, the positive expression rate of Beclin1 in CPA group were lower than those in PA group and control group, the positive expression rate of p53 in CPA group were lower than those in PA group and control group, and the CPA group was higher than the PA group（P〈0.05）. The ex-pression of Beclin1 and p53 in group CPA were related to TNM staging and lymph node metastasis, the positive rate of Beclin1 in TNM stage for III-IV patients was lower than that in I-II stage, the positive expression rate of p53 was higher than that in I-II stage, the positive rate of Beclin1 in patients with lymph node metastasis was lower than that of patients without lymph node metastasis, the positive expression rate of p53 was higher than that of the patients without lymph node metastasis, the differences were statistically significant（P〈0.05）. The expression of Beclin1 and p53 in group CPA were not related to age, sex, tumor diameter and invasiveness（P〉0.05）.Spearman correlation analysis showed that the expression of Beclin1 and p53 in CPA patients were negatively correlated（r=-0.839, P=0.000）. Conclusion： The expression of Beclin1 is low abnormal and p53 is high abnormal in PA and CPA,the expression of Beclin1 and p53 in patients with CPA are related to TNM staging and lymph node metastasis, and the expression of the two is negatively correlated.