当归对实验性血虚便秘模型小鼠结肠组织形态和黏液分泌的影响

Effect of Angelica sinensis radix on colonic morphology and mucus secretion of experimental XuexuBianmi model mice

目的:观察当归对实验性血虚便秘模型小鼠的治疗作用及其对小鼠结肠组织形态和黏液分泌的影响。方法:60只KM小鼠,雌雄各半,随机分为6组(n=10):正常对照组、血虚便秘组、阳性对照组、当归高、中、低剂量组。除正常对照组小鼠外,其余各组小鼠灌胃给予复方地芬诺酯(DNP),皮下注射乙酰苯肼(APH),腹腔内注射环磷酰胺(CPA),复制血虚便秘小鼠模型。从实验的第14天起,当归组小鼠灌胃给予不同剂量的当归煎液(16.67,8.33,4.17 g/kg),阳性对照组给予常通舒颗粒(5 g/kg),血虚便秘模型组和正常对照组小鼠给予等体积生理盐水,每日1次,连续28 d。观察小鼠一般状态,检测首粒黑便排出时间(FBDT)、粪便和结肠含水量,取结肠组织进行HE和AB-PAS染色,观察小鼠结肠组织形态变化和黏液分泌情况。结果:与正常对照组比较,血虚便秘组小鼠出现血虚和便秘体征,FBDT显著延长、粪便和结肠含水量显著降低(P<0.01),结肠黏膜和大肠腺萎缩、黏膜层变薄(P<0.01),黏液分泌减少。与血虚便秘模型组比较,3个剂量的当归均能改善血虚便秘体征,显著缩短FBDT(P<0.05,P<0.01),显著升高小鼠结肠含水量(P<0.05,P<0.01);高、中剂量的当归能显著升高小鼠粪便含水量(P<0.05); 3个剂量当归均能改善血虚便秘模型小鼠的结肠黏膜和大肠腺萎缩,增加结肠黏液分泌,改善结肠润滑功能。结论:当归可改善结肠黏膜萎缩、增加结肠黏液分泌,从而软化粪便促进粪便排出。

Objective： To study the effects of Angelicaesinensis radix（ Danggui） decoction on the therapeutic action and the colonic morphology and mucus secretion in Xuexu Bianmi model mice. Methods： Sixty Kunming mice,maleandfemaleinhalf,were randomly divided into six groups according to gender and weight（ n = 10） ： normal control group,Xuexu Bianmi model group,positive control group,and high-dose,middle-dose and low-dose Danggui groups. Except the normal control group,the mice in the rest groups were orally administrated with diphenoxylate（ DPN） and subcutaneously injected with acetylphenyhydrazine（ APH） and intraperitoneally injected with cyclophosphamide（ CPA） to copy Xuexu Bianmi model. Fromthe 14 th day,the mice in Danggui groups were orally administered with different doses of Danggui decoction（ 16.67、8.33、4.17 g/kg）,the mice in positive control group were orally administered with Changtongshu granule（ 5 g/kg）,the mice in Xuexu Bianmi model group were administered with normal saline（ NS） at the same volume（ 10 ml/kg）,once a day for consecutive 28 days. The general status were observed,the first black defecation time（ FBDT）,the water content in stool and in colon in mice of all groups were tested. And the colon tissue was stained with hematoxylin-eosin（ HE） and AB-PAS to observe the changes of colonic morphology and the mucus secretion. Results： Compared with the normal control group,there appeared the Xuexu Bianmi syndromes in model group as follows,the defecation time（ FBDT） was significantly prolonged,the water contentinstool and in colon were decreased（ P〈0.01）,the colonic mucosa and gland were atrophied,mucous membrane layer was thinned（ P〈0. 01）,mucus secretion was decreased. Compared with the Xuexu Bianmimodel group,the Xuexu and Bianmi syndromes were improved,the FBDT was significantly shortened（ P〈0.05,P〈0.01）,the water content of colon was increased in the three groups of Danggui decoction（ P〈0.05,P〈0.01）. The water content of the stool was obviouslyincreased in groups of 16.67 and 8.33 kg.d Danggui decoction（ P〈0.05）. The atrophy of colon mucosa and gland was improved,the mucus secretion was increased,and the colon lubrication function was improved in the three groups in different doses of Danggui decoction. Conclusion： Dangguican improve the mucosal atrophy,and increase the secretion of colonic mucus,then the stool is softened and promoted to discharge.