禹州漏芦总黄酮最佳提取工艺及抗氧化活性研究

The optimum extraction technology of flavonoids from Radix Echinopsis and its antioxidant activity

禹州漏芦是一种传统中药,目前国内外对禹州漏芦成分和作用研究较多,而对其总黄酮活性物质的提取工艺及抗氧化活性研究未见报道。本研究首先探讨了3种不同提取方法对禹州漏芦总黄酮得率的影响,并对得率最高的提取方法通过单因素及正交实验进行工艺优化,以及提取物进行抗氧化活性测定,以期为将来禹州漏芦的应用提供理论支持。结果表明,酶法-超声波辅助半仿生法提取总黄酮得率最高,此方法的最佳提取工艺为:乙醇浓度50%,料液比1∶75(g/mL),提取时间120 min,纤维素酶用量2%,提取温度50℃和超声波功率140 W。在此最佳提取工艺下,总黄酮得率为12.83%。极差分析表明,6种因素中提取温度对黄酮提取率影响最大,其次是酶含量,超声波功率影响最小。抗氧化活性结果表明:与VC相比,禹州漏芦总黄酮对ABTS+清除能力更强,但对·OH自由基清除能力和Fe^(3+)的还原能力相对较弱。

Radix Echinopsis is a kind of traditional Chinese medicine. Most studies focus on the constituents and role of Radix Echinopsis. However, the extraction process and antioxidant activity of Radix Echinopsis flavonoids have not been reported yet. This study explored the effect on the content of flavonoids by three different extraction methods, and optimizing the extraction process using single factor and orthogonal experiment. The antioxidant activity of extracts was also studied. The result showed that the extraction rate of total flavonoids from semi-bionic enzyme assisted ultrasonic extraction was the highest. The optimal extraction conditions for flavonoids were - 50% ethanol concentration, solid to liquid ratio 1 ： 75 （g/mL）, 2% enzyme content and ultrasonic extraction time 60 min, extraction temperature 50℃ and ultrasonic power 140 W. Under the above optimal extraction conditions, the extraction rate was 12.83%. The order of six influence factors on extraction rate of flavonoids was extraction temperature, followed by enzyme content, and the least influence factor was ultrasonic power. The flavonoids from Radix Echinopsis had higher scavenging activity on ABTS compared with VC under the same concentration. However, it was weaker on scavenging. OH radical as well as Fe^3＋ reducing power.