2017年深圳地区冬季诺如病毒基因分型与流行病学分析

Genotypes and epidemiological characteristics of Noroviruses in Shenzhen during the winter of 2017

目的对深圳地区2017年冬季诺如病毒（Norovirus，NoV）流行株基因型、流行病学特点及同源性进行分析和研究。方法通过提取313份粪便样品的核酸后利用NoV特异性引物进行逆转录PCR（RT．PCR），将阳性PCR产物测序。根据样品测序序列及NoV参考株序列，利用软件Mega4．1及ClustMW构建系统发生树。结果NoV阳性样品共26份且均为GⅡ．4型，分别与GⅡ．4（KY407156）、GⅡ．4（KY580757）及GⅡ．4（KX372682）具有较高的同源性。此外，88．46％的NoV样品与本地区历年流行株同源性较低，且46．15％与我国其他地区流行株存在差异。结论2017年冬季深圳地区诺如病毒流行株以GⅡ．4型为主，且其在流行性及同源性上具有自身的特点．在当地公共卫生工作中需加强关注。

Objective To study the genotypes, epidemiological characteristics and homology of Noroviruses （NoV） circulating in Shenzhen in the winter of 2017. Methods RT-PCR was performed using Nov-specific primers after extracting viral genome from 313 fecal samples. Positive RT-PCR products were then sequenced. Phylogenetic trees were constructed based upon the gene sequences of isolated and reference NoV strains using Mega 4.1 and Clustal W software. Results There were 26 NoV-positive samples and all belonged to G Ⅱ. 4 subtype. These strains shared high homologies with G Ⅱ.4 （KY407156） , G Ⅱ. 4 （KY580757） and G U. 4 （KX372682）. Phylogenetic analysis also suggested that 88.46% of them had a lower homology with the NoV strains isolated in the same area in recent years and 46.15% were different from the epidemic strains in other provinces of China. Conclusion NoV G Ⅱ.4 was the epidemic strain in Shenzhen during the winter of 2017. More attention should be paid to it from the local public health authori- ties considering its owned characteristics in epidemic and homology.