Hp感染与胃癌患者SOX2和VEGF的相关关系及研究意义

The correlation relationship and research meaning between Hp infection and SOX2,VEGF in patients with gastric cancer

[目的]研究幽门螺旋杆菌(Hp)感染与胃癌患者SOX2、血管内皮生长因子(VEGF)的相关关系。[方法]以2016年2月~2018年2月于我院接受手术治疗的胃癌患者80例为研究对象,分别采用酶联免疫吸附法检测胃癌组织与癌旁正常组织中HP感染情况,通过免疫组化SP法检测SOX2、VEGF阳性率情况,并分析上述三项指标与胃癌患者临床病理特征的关系,予以相关性分析。[结果]胃癌组织HP、VEGF阳性率分别为71.25%(57/80)、76.25%(61/80),高于癌旁正常组织的32.50%(26/80)、26.25%(21/80),而SOX2阳性率为58.75%(47/80),低于癌旁正常组织的91.25%(73/80),差异均有统计学意义(均P<0.05)。肿瘤直径≥5cm、TNM分期为Ⅲ~Ⅳ期、低分化、浸润程度浆膜外胃癌患者的HP阳性率分别为87.04%(47/54)、90.00%(45/50)、92.31%(36/39)、84.31%(43/51),均高于肿瘤直径<5cm、TNM分期为Ⅰ~Ⅱ期、中高分化、浸润程度浆膜内的38.46%(10/26)、40.00%(12/30)、51.22%(21/41)、48.28%(14/29),差异均有统计学意义(均P<0.05)。TNM分期为Ⅲ~Ⅳ期、低分化、浸润程度浆膜外胃癌患者的SOX2阳性率分别为44.00%(22/50)、38.46%(15/39)、43.14%(22/51),均低于TNM分期为Ⅰ~Ⅱ期、中高分化、浸润程度浆膜内的83.33%(25/30)、78.05%(32/41)、86.21%(25/29),差异均有统计学意义(均P<0.05)。肿瘤直径≥5cm、TNM分期为Ⅲ~Ⅳ期、低分化、浸润程度浆膜外胃癌患者的VEGF阳性率分别为88.89%(48/54)、92.00%(46/50)、89.74%(36/39)、84.31%(43/51),均高于肿瘤直径<5cm、TNM分期为Ⅰ~Ⅱ期、中高分化、浸润程度浆膜内的50.00%(13/26)、50.00%(15/30)、63.41%(26/41)、62.07%(18/29),差异均有统计学意义(均P<0.05)。经Preason相关性分析可得:HP感染与胃癌患者SOX2表达呈负相关关系(r=-0.743,P=0.000),与VEGF表达呈正相关关系(r=0.718,P=0.000)。[结论]Hp感染与胃癌患者SOX2、VEGF存在密切相关,临床工作中可通过对联合检查三项指标表达情况,从而有助于胃癌的早期诊断以及预后评估。

[Objective]To study the correlation relationship between helicobacter pylori（Hp）infection and SOX2,vascular endothelial growth factor（VEGF）in patients with gastric cancer.[Methods]From February 2016 to February 2018 in our hospital accepted surgical treatment of 80 cases of gastric cancer patients as the research object,HP infection condition in gastric cancer tissue and normal tissue adjacent tocarcinoma were detected by enzyme-linked immunosorbent method,SOX2,VEGF positive rate were detected by immunohistochemical SP method,and relationship between the above three indicators and the clinical pathological features of gastric cancer patients was analyzed,the correlation analysis were done.[Results]The gastric cancer group of HP,VEGF positive rate was 71.25%（57/80）,76.25%（61/80）,which was higher than the normal tissue adjacent to carcinoma of 32.50%（26/80）,26.25%（21/80）,and SOX2 positive rate was 58.75%（47/80）,which was less than the normal tissue adjacent to carcinoma of 91.25%（73/80）,differences were statistically significant（all P〈0.05）.Tumor diameter≥5 cm,TNM stage wasⅢ~Ⅳ,low degree of differentiation,infiltration serosa outside of HP positive rate of gastric cancer patients were 87.04%（47/54）,90.00%（45/50）,92.31%（36/39）,84.31%（43/51）,which were higher than tumor diameter5 cm,TNM staging was Ⅰ ~ Ⅱ high degree of differentiation,infiltration serous inside of38.46%（10/26）,40.00%（12/30）,51.22%（21/41）,48.28%（14/29）,the difference was statistically significant（all P〈0.05）.TNM stage was Ⅲ ~ Ⅳ,low degree of differentiation,infiltration serosa outside of SOX2 positive rate of gastric cancer patients were 44.00%（22/50）,38.46%（15/39）,43.14%（22/51）,which were lower than the TNM staging wasⅠ~Ⅱ,high degree of differentiation,infiltration serous inside of 83.33%（25/30）,78.05%（32/41）,86.21%（25/29）,differences were statistically significant（all P〈0.05）.Tumor diameter ≥5 cm,TNM stage was Ⅲ ~ Ⅳ,low degree of differentiation,infiltration serosa outside of VEGF positive rate of gastric cancer patients was 88.89%（48/54）,92.00%（46/50）,89.74%（36/39）,84.31%（43/51）,which were higher than tumor diameter5 cm,TNM staging wasⅠ~Ⅱ,high degree of differentiation,infiltration serous inside of 50.00%（13/26）,50.00%（15/30）,63.41%（26/41）,62.07%（18/29）,the difference was statistically significant（all P〈0.05）.According to Pearson correlation analysis,HP infection was negatively correlated with the expression of SOX2 in patients with gastric cancer（r=-0.743,P=0.000）,and was positive correlation with VEGF expression（r=0.718,P=0.000）.[Conclusion]Hp infection is closely related to SOX2,VEGF in patients with gastric cancer.In clinical work,the expression of three indexes can be used for early diagnosis and prognosis evaluation of gastric cancer.