豫西南地区新发现HIV/AIDS病人外周血淋巴细胞亚群及WB带型分析

Analysis of lymphocyte subsets in peripheral blood and WB band type of new HIV carriers and AIDS patients in the southwest of Henan province

目的分析豫西南地区新发现的艾滋病病毒（HIV）感染者/艾滋病（AIDS）病人（简称HIV/AIDS病人）的外周血淋巴细胞亚群和实验室蛋白印迹条带（WB）带型,为平顶山市的艾滋病防控寻找依据。方法选取2014年1月至2016年12月,平顶山市疾病预防控制中心艾滋病确证实验室检测的580例HIV/AIDS病人为研究对象,设置AIDS病人（A组）、HIV感染者（B组）、健康体检者（C组）,统计分析三组外周血淋巴细胞亚群[CD4^＋T淋巴细胞（简称CD4细胞）、CD8^＋T淋巴细胞（简称CD8细胞）、CD4/CD8]计数、比值和WB带型。结果 B组108例HIV感染者,CD4细胞计数、CD4/CD8比值低于137例健康体检者（对照组）,CD8细胞计数高于对照组（P〈0.05）;A组472例AIDS病人CD4细胞和CD8细胞计数、CD4/CD8比值低于B组,差异有统计学意义（P〈0.05）。将A组和B组按照性别（男女）、年龄（〉50岁,≤50）再分组,按CD4细胞和CD8细胞计数、CD4/CD8比值对比,差异均无统计学意义。A组、B组WB全带型和次全带型发生率对比分析,差异有统计学意义;按照WB全带型和次全带型分析,A、B两组CD4细胞和CD8细胞计数对比,差异均有统计学意义;A组WB全带型与次全带型组内分析,CD4细胞计数、CD4/CD8比值对比,差异均有统计学意义,CD8细胞计数对比,差异无统计学意义;B组全带型与次全带型组内分析,CD4/CD8比值对比,差异有统计学意义。结论豫西南地区新发现病例以艾滋病病人为主,淋巴细胞破坏较重,HIV感染者次全带的发生率显著高于艾滋病病人。

Objective To analyze the changes of peripheral blood lymphocyte subsets in HIV/AIDS patients in the southwest of Henan province,so as to find the basis for AIDS prevention and control.Methods From Jan 2014 to Dec2016,there were 580 cases confirmed as HIV infected by Pingdingshan city CDC.Three groups were set up including AIDS patients group（A）,HIV carriers group（B）and healthy people（C）.The peripheral blood lymphocyte subsets（CD4＋,CD8＋,CD4＋/CD8＋）and WB band type were tested and then statistical analysis made accordingly.Results Group B（108 cases）had lower of CD4 cells,and CD4/CD8 cells than group C（137 cases）and CD8＋ count was higher than group C（P〈0.05）.The CD4＋,CD8＋and CD4/CD8 counts of group A（472 cases）were lower than those of group B（P〈0.05）.Group A and group B were regrouped according to gender,and age（〉50 years,≤50）and there was no statistical difference between two groups in CD4^＋,CD8^＋ and CD4^＋/CD8^＋counts.The difference of incidence rate was statistically significant between WB full band and subtotal band in group A and B.According to WB full band and subtotal band,group A and B had statistically significant difference of CD4^＋,CD8^＋ counts;the difference of CD4^＋,CD4^＋/CD8^＋counts between WB full band and subtotal band in group A was statistically significant,as well as group B.Conclusion The newly discovered cases in the southwest of Henan province are mainly AIDS patients,with serious lymphocyte damage.The incidence for WB subtotal band of HIV carriers is significantly higher than that of AIDS patients.