合并带状疱疹病毒感染对HIV-1感染者外周血中HIV-1DNA的影响

Impact of varicella-zoster virus coinfection on the decay of HIV-1DNA in the peripheral blood of HIV-1 infected patients

目的观察艾滋病病毒1型（HIV-1）合并带状疱疹病毒（VZV）感染者,在高效抗反转录病毒治疗（HAART）前后外周血中HIV-1脱氧核糖核酸（DNA）水平的变化。方法选取慢性期HIV-1合并VZV感染者11例作为试验组（VZV＋）,46例未合并VZV感染者作为对照组（VZV-）。对0周和抗病毒治疗48周时感染者外周血进行HIV-1DNA的定量检测。结果基线时,VZV＋组HIV-1DNA水平为（3.40±0.36）Log10拷贝/10^6外周血单个核细胞（PBMCs）,明显高于VZV-组的（3.04±0.44）Log10拷贝/10^6 PBMCs（P=0.014）。抗病毒治疗48周后,VZV＋组HIV-1DNA水平为（3.19±0.33）Log10拷贝/10^6 PBMCs,较基线时未出现明显的下降（P=0.182）;VZV-组HIV-1DNA水平为（2.56±0.46）Log10拷贝/10^6 PBMCs,较基线时明显下降（P〈0.001）,并且显著低于VZV＋组（P〈0.001）。治疗前和治疗后的HIV-1DNA水平均与基线病毒载量呈正相关,与基线CD4细胞计数、辅助性T细胞/抑制性T细胞比例呈负相关。结论 HIV-1合并VZV感染者具有较高的基线HIV-1DNA,并且在有效地抗病毒治疗之后仍然高于未合并VZV感染者。

Objective To observe the impact of varicalle-zoster virus（VZV）coinfection on the decay of HIV-1 DNA in HIV-1 infected patients before and after highly active antiretroviral therapy（HAART）.Methods 11 HIV-1 chronically infected patients co-infected with VZV were recruited as the study group and 46 HIV-1 infected patients without co-infection as controls.HIV-1 DNA was quantified with peripheral blood at week 0 and 48.Results Compared with the VZV-group,the baseline HIV-1 DNA of the VZV＋ group was much higher（3.40±0.36 vs3.04±0.44 Log10 copies/10^6 PBMCs,P=0.014）.At week 48 on HAART,the level of HIV-1 DNA of the VZV＋group was 3.19±0.33 Log10 copies/10^6 PBMCs,without significant decrease（P=0.182）.As for the VZV-group,HIV-1 DNA dramatically decayed to 2.56±0.46 Log10 copies/10^6 PBMCs,much lower than baseline level（P〈0.001）and the VZV＋ group（P〈0.001）.Both the levels of HIV-1 DNA at baseline and after treatment were positively correlated to baseline viral load,but negatively correlated to CD4 cell count and Th/Ts ratio.ConclusionHIV-1 patients co-infected with VZV present higher HIV-1 DNA level no matter before or after effective HAART.