500份HIV 1/2抗体蛋白印迹试验阳性样本的带型分析

Banding pattern analysis of 500 HIV1/2 antibody positive samples tested by western blot

目的分析2010—2017年丹东地区艾滋病病毒（HIV）1/2抗体蛋白印迹试验（WB）阳性样本的带型分布规律,为制定该地区切实有效的艾滋病防控措施提供科学依据。方法采用WB对2010—2017年500份HIV-1抗体筛查阳性样本进行确证检测,并对WB带型进行统计分析。结果 500份HIV-1抗体阳性样本中,WB带型组合为全带型和次全带的占90.0%（450份）;抗env、抗pol基因编码的抗体条带和抗gag基因编码的p24检出率均超过93.0%,p55和p17检出率分别为68.8%和85.2%。感染者以男性居多,p24在不同性别中检出率的差异有统计学意义（χ^2=6.706,P〈0.05）;抗env基因编码的抗体在各个年龄组的检出率差异均无统计学意义（P〉0.05）,抗pol基因编码的抗体在各个年龄组的检出率差异均有统计学意义（P〈0.05）,抗gag基因编码的抗体除p55外,p24和p17在各个年龄组的检出率差异均有统计学意义（P〈0.05）。结论该地区大部分感染者在艾滋病期前被及时发现,中青年人群为该地区HIV感染的高危人群,应重点防控。

Objective To analyze the pattern distribution of HIV-1/2 antibody positive samples tested by western blot（WB）in Dandong city,to provide effective measures for HIV/AIDS prevention and control.Methods We used WB to detect 500 HIV-1 antibody positive samples from 2010 to 2017,and statistical analysis was made to know the WB belt types.Results Out of 500 HIV-1 antibody positive samples,WB band was mainly composed of full band and sub band,accounting for 90.0%（450）.The detection rate of the antibodies encoded by anti-envgene and anti-pol genes was more than 93%,as well as p24 encoded by anti-gag gene.The detection rate of p55 and p17 was 68.8% and 85.2% respectively.Patients were mainly male,and the difference of p24 detection rate in different genders was statistically significant（χ^2=6.706,P〈0.05）.The detection rate of anti-Env genes encoded antibodies was not statistically significant in all age groups（P〉0.05）,but the antibodies encoded by anti-pol genes were statistically different（P〈0.05）.The differences of detection rate of antibodies encoded by anti-gag genes of p24 and p17,in spite of p55,were statistically significant in all age groups（P〈0.05）.Conclusion Most of the people in Dandong are early detected,showing the current HIV/AIDS prevention and control measures are effective.Young and middle-aged people are at high risk of HIV infection,and are our priority groups for HIV/AIDS prevention and control.