SPE-HPLC法测定松花江哈尔滨段水样中11种抗生素

Determination of 11 Antibiotics in Harbin Section of Songhua River by SPE-HPLC

应用SPE-HPLC法检测松花江哈尔滨段4种喹诺酮类、3种磺胺类和4种大环内酯类抗生素的含量。实验结果表明,固相萃取小柱的富集效果好,抗生素在10～2 000μg/L浓度范围内线性关系良好,精密度、重现性、回收率良好,符合试验要求。测定结果表明,松花江哈尔滨段选取的四个监测断面均存在抗生素的污染,朱顺屯断面主要是喹诺酮类抗生素的污染,阿什河口下和呼兰河口下断面主要是磺胺类和大环内酯类抗生素的污染,大顶子山断面仅在6月份检出红霉素。经分析可知抗生素污染主要来自沿岸企业废水和生活污水,同时受径流量、流速因素以及气候的影响。

The content of four quinolones, three sulfonamides and four macrolide antibiotics in the Harbin section of Songhua River was detected by SPE-HPLC. The experimental results showed that the solid phase extraction cartridge had a good enrichment effect. A good linear relationship had been gained in the antibiotics concentration range of 10 -2 000 μg/L, and the precision, reproducibility and recovery rate all reached the test requirements. The test results showed that there were antibiotic pollution in the four monitoring sections selected in the Harbin section of Songhua River. Quinolone antibiotics were the main pollutarlts in Zhushuntun section. Suffonamides and macrolides were the main pollutants in Ashihek- ouxia and Hulanhekouxia sections. Erythromycin were detected in Dadingzishan section only in June. Ac- cording to the analysis results, the pollution of antibiotics mainly came from coastal enterprise wastewater and domestic sewage, and was also affected by other factors such as runoff, flow rate factors and climate.