固相萃取-高效液相法测定黄酒中多酚

Application of SPE-HPLC for Determination of Phenolic Compounds in Chinese Rice Wines

为了寻找一种更高效、富集能力更强,更具有针对性的前处理方式,解决黄酒多酚测定时存在的杂质多、目标物含量低、定量不准确的问题,采用固相萃取小柱对黄酒进行前处理,就流动相条件、固相萃取小柱类型、洗脱剂类型、洗脱剂体积等因素对9种多酚的回收率影响做了评价比较,并运用该方法对不同甜型的绍兴黄酒中多酚质量浓度进行精确测定,结果表明:采用Poly-Sery HLB SPE小柱,甲醇为洗脱剂,洗脱体积为3倍上样体积时9种多酚回收率最高,均在87.3%～103.8%之间,相对标准偏差小于3.2%。不同甜型的绍兴黄酒中多酚以儿茶素和丁香酸为主,干型黄酒、半干型黄酒中多酚质量浓度低于甜型黄酒和半甜型黄酒。该方法具有快速、简便、准确的优点,可用于黄酒等酒类的多酚测定。

In order to search for a rapid technique for the isolation of polymeric polyphenols from Chinese rice wine for solving the problem that accurately measured the polyphenols in Chinese rice wine because of the impurity interference and low target content, in this paper,the effects of recoveries for the 9 kinds ofpolyphenol compounds were evaluated by the condition of mobile phase and SPE cartridge types as well as the composition of eluent and the volume of eluent by a high-performance liquid chromatographic method with ultraviolet detection,in the end,using this method for determination polyphenol content in different sweet type of shaoxing Chinese rice wine for precise measurement. Results show that the recovery were between 87.3%-103.8% and the RSD less than3.2% when the Poly-Sery HLB SPE cartridges were used and methanol as eluent and the elution volume for 3 times on the sample volume, catechin and Syringic acid has the highest content in different sweet type of shaoxing Chinese rice wine, the content of polyphenol in dry type and half dry type is lower than the sweet type and half sweet type Chinese rice wine .This SPE method is simple,rapid,and accurate. It can be satisfactorily applied to the determination of polyphenol compounds in wine drinks.