藏药黑果枸杞水提物对运动疲劳所致氧化应激的影响

Effects of Lycium Ruthenicum Murr. Aqueous Extract on Oxidative Stress Induced by Exercise Fatigue

目的研究藏药黑果枸杞水提物对运动疲劳所致氧化应激的影响。方法 150只小鼠随机分为3组,分别是泳前组、泳后即刻组和泳后恢复30 min组,每组50只。每组再分为5小组,每组10只,分别是阳性组、空白组和黑果枸杞高、中、低剂量组,灌胃给药30 d后测定小鼠泳前、泳后即刻和泳后恢复30 min后血清中丙二醛(malondialdehyde,MDA)、脂质过氧化物(lipidperoxide,LPO)、活性氧物质(reactiveoxygenspecies,ROS)含量及超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活性。同时采用体外自由基发生体系,评价藏药黑果枸杞对1,1-二苯基-2-三硝基苯肼(DPPH)、羟基自由基(·OH)、超氧阴离子(O2-·)的清除力。结果黑果枸杞不但可以明显降低泳后及泳后恢复组小鼠血清中MDA、LPO、ROS含量,增加SOD、CAT、GSH-Px活性,而且可以减少DPPH、·OH、O2-·的产生。结论藏药黑果枸杞可明显缓解由于运动疲劳所引起的氧化应激反应。

OBJECTIVE To study the effects of Lycium ruthenicum Murr. aqueous extract on oxidative stress induced by exercise fatigue. METHODS One hundred and fifty mice were randomly divided into 3 groups, 50 in each group： no swimming group, immediate after swimming group and restore 30 min after swimming group. Mice in each group were subdivided into 5 groups of 10 each： positive control group, blank control group and Lycium ruthenicum Murr. low, medium and high dose group. After 30 d of intragastric administration, the contents of MDA, LPO and ROS, and the activities of SOD, CAT, GSH-Px in serum were measured. At the same time, the in vitro free radical generation system was used to evaluate the scavenging power of Lycium ruthenicum Murr. on 1,1-diphenyl-2-trinitrophenylhydrazine（DPPH）, ·OH and O2-·. RESULTS Lycium ruthenicum Murr. could not only reduce the contents of MDA, LPO and ROS in the serum of mice after swimming and 30 min after swimming significantly, but also increase the activities of SOD, CAT and GSH-Px. Moreover, it could reduce the production of DPPH, ·OH and O2-· radicals. CONCLUSION Lycium ruthenicum Murr. can alleviate the oxidative stress caused by exercise fatigue obviously.