摘要
Aggregation-induced emission luminogens （AIEgens） are fluorescent agents thatare ideal for bioimaging and have been widely used for organeUe targeting, cellularmapping, and tracing. Owing to their promising characteristics, AIEgen-basednanoparticles have recently been used for the stimulated emission depletion（STED） super-resolution imaging of fixed cells. In the present study, and for thefirst time, we used an AIEgen for dynamic STED nanoscopic imaging of a specificorganelle in live cancer cells. TPA-T-CyP is a synthetic red＆NIR-emittingluminogen with AIE features that can spontaneously and specifically aggregateon mitochondria without the need for encapsulation or surface modification.The STED efficiency of aggregated TPA-T-CyP can reach more than 80%, andsuper-resolution imaging of TPA-T-CyP-stained mitochondria in live HeLa cellsis possible, with a lateral spatial resolution of 74 nm. We found that TPA-T-CyPenabled the dynamic visualization of mitochondria, and the motion, fusion, andfission of mitochondria were dearly observable on a super-resolution scale.AIEgen-based super-resolution organelle visualization has great potential formany basic biomedical studies.
基金
This work was supported by the Zhejiang Provincial Natural Science Foundation of China （LR17F050001）, the National Natural Science Foundation of China （Nos. 61735016 and 51622305）, and the National Basic Research Program of China （Nos. 2013CB834704 and 2015CB856503）, the PCSIRT （No. IRT13023）, the Science ＆ Technology Project of Tianjin of China （No. 15JCYBJC29800）, the China Postdoctoral Science Foundation （No. 2016M601252）, and the 111 project （B08011）.
