益气养阴清热方对糖尿病KKAy小鼠血清胰岛素及TNF-α、IL-6的影响

Effects of yiqi yangyin qingre formula on serum insulin,TNF-α and IL-6 in the KKAy mice with diabetes

目的观察益气养阴清热方对糖尿病KKAy小鼠血清胰岛素及肿瘤坏死因子-α(Tumor Necrosis Factor-alpha,TNF-α)、白细胞介素6(Interleukin-6,IL-6)的影响。方法 5周龄雄性SPF(Specificpathogenfree,SPF)级自发性2型糖尿病KKAy小鼠50只,随机分为模型组、二甲双胍组(0.2 g/kg)、益气养阴清热方高剂量组(2 g/kg)、益气养阴清热方中剂量组(1 g/kg)、益气养阴清热方低剂量组(0.5 g/kg),每组10只。取同周龄雄性SPF级C57BL/6J小鼠10只为正常组。正常组和模型组灌服生理盐水。干预8周后,检测各组小鼠体重、血糖、血清胰岛素、TNF-α、IL-6水平,计算小鼠胰岛素抵抗指数(Homa-Insulin,HOMA-IR)。结果与正常组比较,模型组小鼠空腹及随机血糖、胰岛素水平、胰岛素抵抗指数明显升高(P<0.01)。干预8周后,与模型组比较,益气养阴清热方高、中、低剂量组小鼠体重、胰岛素水平、HOMA-IR指数均明显下降(P<0.01)。益气养阴清热方高、中剂量组能降低TNF-α水平,剂量呈依赖性(P<0.01),其中益气养阴清热方高剂量组效果最优;益气养阴清热方高、中、低剂量组均能使IL-6水平降低,差异有统计学意义(P<0.05),剂量呈依赖性。结论益气养阴清热方能改善糖尿病小鼠胰岛素抵抗,降低空腹及随机血糖,其机制可能是通过抑制炎症反应来实现。

Objective To observe the effects of yiqi yangyin qingre formula on serum insulin,TNF-α and IL-6 in the KKAy mice with diabetes. Methods A total of 50 KKAy male SPF mice of spontaneous type 2 diabetic mellitus（ T2 DM）,at the age of 5 weeks. They were randomized into a model group,a metformin group（ 0. 2 g/kg）,a high-dose group of yiqi yangyin qingre formula（ 2 g/kg）,a middle-dose group（ 1 g/kg） and a low-dose group（ 0. 5 g/kg）,10 mice in each one. Additionally,10 C57 BL/6 L male SPF mice,at the age of 5 weeks were collected in the normal group. The physical saline was used in lavage in the model group and the normal group. In 8 weeks of intervention,the body weight,blood glucose,serum insulin,TNF-α and IL-6 were determined in each group. The HOMA-IR was calculated. Results Compared with the normal group,the fasting and random blood glucose,insulin and the insulin resistance index were increased obviously in the model group（ P〈0. 01）. After 8-week intervention,compared with the model group,the body weight,insulin and HOMA-IR index were all reduced obviously in the high-dose,middle-dose and low-dose groups of yiqi yangyin qingre formula（ P〈0. 01）. TNF-α was reduced in the high-dose and middle-dose groups,presenting the dose dependence（ P〈0. 01）,in which,the effect was the best in the high-dose group. IL-6 was all reduced in the high-dose,middle-dose and low-dose groups,indicating the significant differences（ P〈0. 05）,presenting the dose dependence. Conclusion Yiqi yangyin qingre formula improves the insulin resistance and reduces the fasting and random blood glucose in the mice of diabetes. The effect mechanism is probably related to the inhibition of the inflammatory reaction.