毛细管电泳电化学发光同步测定红花石蒜中的伪石蒜碱、石蒜碱和高石蒜碱

Capillary electrophoresis with end-column electrochemiluminescence for simultaneous determination of pseudolycorine,lycorine and homolycorine in lycoris radiata

以聚乙烯吡咯烷酮(PVP)为分离缓冲溶液添加剂,应用毛细管电泳-电化学发光同步测定了红花石蒜中伪石蒜碱、石蒜伦碱、高石蒜碱3种生物碱。对分析方法的检测电位、分离电压、进样电压、进样时间、分离缓冲溶液及添加剂用量等实验条件进行了实验考察。在选定实验条件下,3种生物碱在12 min内能实现较好分离。伪石蒜碱、石蒜碱、高石蒜碱的检出限分别为0. 012,0. 016,0. 021μg/mL。将本法应用于石蒜中上述3种生物碱的测定,回收率分别为101. 6%,99. 6%,98. 9%,结果令人满意。

Polyvinylpyrrolidone was added into the running buffer as an additive to obtain the absolute separation, a electrochemilumineseenee method for simultaneous determination of pseudolyeorine, lyeorine and homolycorine in lycoris radiata based on capillary electrophoresis with ultrasonic assisted extraction was developed. Several parameters affecting the determination were studied, including additive, detection potential, separation voltage, injection voltage and injection time, the pH and concentration of running buffer. Under the optimum experimental conditions, the absolute separation of the three alkaloids was obtained within 12 s and the limits of detection were 0. 012,0. 016 and 0. 021 μg/mL for pseudolyeorine, lycorine and homolycorine （ S/N= 3 ） , respectively. This method was successfully applied to determine the three alkaloids in lyeoris radiata with the recoveries were 101.6%, 99.6% and 98.9% for pseudolycorine, lycorine homolycorine, respectively.