2013-2017年徐州市儿童腺病毒流行特征与基因分型

Epidemiological characteristics and genotypes of human adenovirus among children in Xuzhou from 2013 to 2017

目的了解2013-2017年徐州市儿童腺病毒感染的流行情况及其分型特征。方法采集2013-2017年在徐州市儿童医院就诊的5177份流感样患儿的鼻或咽拭子。采用实时荧光PER方法进行腺病毒的检测，对阳性标本扩增其六邻体基因并测序进行型别分析。结果5177例患儿中共检出174例腺病毒阳性，检出率为3．36％。其中，3-6岁年龄组的患儿检出率最高，为5．30％（111／2095）；全年各月份均有腺病毒检出，其中5月和6月为检出高峰，两月合计检出的腺病毒例数占检出总数的43．10％（75／174）。型别分析结果显示，本次研究共检出B、C和E共3组10种型别腺病毒，其中B组腺病毒所占比例最高，为80．46％（140／174），包括腺病毒3型107例，7型20例，55型8例和14型5例；其次为C组，占12．07％（21／174），包括1型13例，2型5例，腺病毒5型、6型和57型各l例；以及E组腺病毒4型，占7．47％（13／174）。结论2013—2017年徐州地区儿童呼吸道疾病腺病毒检出率为3．36％，感染以腺病毒3型和7型为主，其次为腺病毒1型和4型等。2013-2014年腺病毒流行以3型和7型为主，2015年后腺病毒3型逐渐成为优势型别。

Objective To study the epidemiological characteristics and genotypes of human adenovirus （HAdv） in Xuzhou from 2013 to 2017. Methods A total of 5 177 swap samples were collected from children with influenza-like illness in Xuzhou Children＇s Hospital during 2013-2017. HAdv was detected by Real-time PCR. Partial sequences of HAdV hexon gene were amplified from the positive samples, sequenced and genotyped. Results Among the 5 177 samples, 174 samples were tested positive of HAdV, and the detection rate was 3.36%. Among them, the highest detection rate was 5.30% （111/2 095） in the 3-6 years old group. Adenovirus was detected in each month, with peaks of detection in May and June, accounting for 43.10% （75/174） of all positive cases. The genotype analysis showed that 10 genotypes of adenoviruses belonged to B, C and E subgroups were detected. The proportion of subgroup B adenovirus was the highest, accounting for 80.46% （140 cases）, including 107 cases of HAdV-3, 20 cases of HAdV-7, 8 cases of HAdV-55 and 5 cases of HAdV-14. The group C accounted for 12.07% （21 cases）, including 13 cases of HAdV-1, 5 cases of HAdV-2, and 1 case each of HAdV-5, 6 and HAdVo57. HAdV-E4 accounted for 7.47% （13 cases）. Conclusions The detection rate of adenovirusamong children with respiratory diseases in Xuzhou was 3.36% from 2013 to 2017. The predominant genotypes of HAdV infection were adenovirus genotypes 3 and 7, followed by genotypes 1 and 4. The prevalence of adenovirus in 2013-2014 were mainly genotype 3 and 7. After 2015, adenovirus genotype 3 gradually became the dominant type.