株洲清水塘工业区周边土壤微生物群落特征

Microbial Communities in Soils of Qingshuitang Industrial District in Zhuzhou

基于Illumina高通量测序技术分析了株洲清水塘工业区周边土壤的微生物群落特征,研究重金属污染对土壤微生物群落的影响.结果表明,土壤微生物群落的相对丰度和多样性变化趋势一致,均随着重金属污染程度增加而减小;稻田土壤平均相对丰度最高的门是变形菌门(49. 56%),其次为绿弯菌门(13. 07%)和酸杆菌门(8. 77%);较高重金属污染程度下伴随着更高丰度的变形菌门、绿弯菌门与更低丰度的硝化螺旋菌门、酸杆菌门; 4组样品的微生物群落结构相似性较高,OTU重叠度达52. 64%,丰度较高的OTU其微生物群落更趋向于相似;结合Spearman相关性分析,Cr与变形菌门显著负相关,与绿弯菌门显著正相关,Cd、Cu、Pb和Zn与硝化螺旋菌门显著负相关.以上结果表明,重金属污染是影响清水塘工业区周边土壤微生物群落结构的重要因素.

To study the effect of heavy metal pollution on microbial communities,microbial diversity and community structure of soils near Qingshuitang industrial district in Zhuzhou,China,were analyzed by Illumina high-throughput sequencing technology. In this study,the microbial diversity and community relative abundance decreased with increased heavy metal pollution level. Proteobacteria（ 49. 56%） were the most abundant phylum in all samples,followed by Chloroflexi（ 13. 07%） and Acidobacteria（ 8. 77%）. The microbial community structures of all samples were similar. The overlap of OTUs was 52. 64%,and the structures of the abundant OTUs subcommunities were more similar than the rare OTUs community structures were. Heavy metals caused increases in Proteobacteria and Chloroflexi and decreases in Acidobacteria and Nitrospirae. According to Spearman＇s correlation,Proteobacteria was significantly negatively correlated with Cr, whereas Chloroflexi was positively correlated with Cr. Cd, Cu, Pb, and Zn were significantly negatively correlated with Nitrospirae. These results showed that heavy metal pollution is an important factor affecting the soil microbial structure in the soil of the Qingshuitang industrial district.