用单克隆抗体检测SLE患者IL－2及IL－6分泌细胞量

Detection of IL-2 and IL-6 producer cells with monoclonal antibodies in SLE patients

ＩＬ－２和ＩＬ－６是机体重要的免疫调节因子。采用单克隆抗体以免疫酶标技术（ＡＰＡＡＰ法）对不同病期ＳＬＥ患者外周血单个核细胞中的ＩＬ－２、ＩＬ－６分泌细胞量进行了检测。结果表明：与正常人比较，活动期患者ＩＬ－２、ＩＬ－６分泌细胞量均升高（Ｐ＜０．０１），且二者间具相关性（ｒ＝０．８８，Ｐ＜０．０５）；非活动期患者ＩＬ－２分泌细胞量升高（Ｐ＜０．０１）而ＩＬ－６分泌细胞量与正常接近。

The IL-2 and IL-6 are important immunomodulating factors of our body.By using monoclonal antibodies and immunoenzyme labelling technique(APAAP method),the IL-2 and IL-6 producer cells were detected in peripheral blood mononuclear cells from patients with active and inactive SLE. The results showed that both of the IL-2 and IL-6 producer cells are significantly increased in patients with active SLE compared with normal(P<0.0 1). They also had positive correlation (r= 0. 88, P<0. 05). In inactive SLF , the number of IL-2 producer cells are higher than that of normal ones while the number of IL-6 producercells did not show difference to controls.