青海省辣椒疫霉菌SSR标记的遗传多样性分析

Genetic Diversity Analysis of Phytophthora capsici SSR Markers in Qinghai Province

本研究采用SSR分子标记法对采集自青海省不同地区的80份辣椒疫霉菌及4份标准菌株进行遗传多样性分析。以采集的84份辣椒疫霉菌为实验材料,筛选出30对特异性高,条带清晰的引物进行辣椒疫霉菌的遗传多样性分析,30对SSR引物扩增的总条带数为127条,多态性条带为114条,多态性检测率为89.7%,PIC范围为0.381～0.837,平均每对引物的多态性信息量为0.594。本研究采用非加权组平均法进行了聚类分析,得出结论:遗传距离主要分布于0.42～0.94这个区间,平均遗传距离是0.62,相似性系数为0.71的时候可以将供试的84份辣椒疫霉菌分成9个大类,84份辣椒疫霉菌表现出了丰富的遗传多样性。实验结果显示,第一聚类中包括了来自6个地区的20个菌株,成为了优势种群。本研究为青海省辣椒疫霉菌抗病育种工作提供了一定的理论依据。

In this study, SSR molecular markers were used to analyze the genetic diversity of 80 strains of P. capsici and 4 standard strains collected from different areas of Qinghai province. 84 samples of P. capsici were selected as the experimental material, and 30 pairs of primers with high specificity and clear bands were screened for genetic diversity analysis of P. capsici. The total number of bands amplified by 30 pairs of SSR primers was 127, in which 114 were polymorphic bands, with the polymorphism rate of 89.7%. The PIC range was 0.381- 0.837, and the average polymorphic information of each pair of primers was 0.594. In this study, cluster analysis was carried out by UPGMA method, the result of which showed that the genetic distance was between 0.42 and 0.94, with an average of 0.62. When the similarity coefficient was 0.71, 84 species ofP. capsici could be divided into nine major categories, and 84 samples ofP. capsici showed abundant genetic diversity. The results indicated that the first cluster included 20 strains from 6 regions, which were the dominant populations. This study laid a theoretical foundation for the disease resistance breeding ofP. capsici in Qinghai province.