HPLC法测定人血清中头孢呋肟浓度

DETERMINATION OF CEFUROXIME IN HUMAN SERUM BY HPLC

本文建立了快速、简便测定人血清中头孢呋肟反相HPLC法,采用5×20mn不锈钢柱内填10um YWG-C_(18)H_(37)17定相,流动相甲醇:水:冰醋酸(28:71:1v/v),流速1.2ml/min,以头孢噻肟为内标物,紫外检测波长290nm,用10％三氯醋酸作蛋白沉淀剂,与血清之比为1:2(v/v)。头孢呋肟的日内回收率为100.34±6.60％,日间回收率为99.69±6.33％。头孢呋肟最低检测限为2ng,最低检测浓度为0.2μg/ml。在0.25～10μg/ml浓度范围内线性良好,相关系数r=0.9999。

A rapid and convenient quantitative method for the detemination of Cefuroxime in human plasma by reversed HPLC was established. ID 5 × 200mm stainless steel column filled with 10 um YWG-C18H37was used. The mobile phase was composed for methane: puritied waters acetic acid (28 : 71 : 1 v/v)with a flow rate of 1. 2ml/min. Claforan was used as a internal standard. The dection was carried out at UV 290nm. A 10% trichloroacetic acid was used for the sedimentation of protein at a ratio of 1: 2(v/v)to serum. The average relative recoveries of within day and between day were 100. 34±6. 60%and 99. 69±6. 33% respectively. Detection limit of cefuroxime was 2ng/ml in plasma. The method kept linear within the range from 0. 25-10μg/ml, and the coefficient of corrlation was 0. 9999.