血浆置换对慢加急性(亚急性)肝衰竭患者外周血CD34^+细胞生物学活性的影响

Effect of plasma exchange on bioactivity of peripheral blood CD34^+ cells in patients with acute-on-chronic (subacute) liver failure

目的研究慢加急性(亚急性)肝衰竭患者血浆置换前后机体内环境的改变对外周血CD34+细胞生物学活性的影响。方法采用含慢加急性(亚急性)肝衰竭患者血浆置换前后血清的培养基体外培养患者外周血CD34+细胞,通过比较2组细胞的生长曲线,及RT-PCR检测细胞表面标志物PK-M2、Integrin-β1、L-PK的表达,免疫荧光检测细胞表面标志Integrin-β1的表达情况,了解血浆置换后机体内环境的改善对外周血CD34+细胞生物学活性的影响。计量资料组间比较采用重复测量资料方差分析。结果血浆置换后组外周血CD34+细胞的增殖明显优于含血浆置换前血清培养组(P <0. 05);血浆置换前组与血浆置换后组均能检测到PK-M2、Integrin-β1,未检测到L-PK。结论血浆置换对肝衰竭患者机体内环境的改善有利于维持外周血CD34+细胞的生物学活性,有助于提高干细胞移植治疗肝衰竭的疗效。

Objective To investigate the effect of improvement in internal environment after plasma exchange on the bioactivity of peripheralblood CD34 ^＋ cells in patients with acute - on - chronic （ subacute）liver failure. Methods The peripheral blood CD34^ ＋ cells from patients with acute - on - chronic （ subacute）liver failure were cultured in vitro using the medium containing with the serum collected before orafter plasma exchange. The growth curves of the two groups of cells were recorded and compared. RT - PCR was used to detect the expression of the cell surface markers PK - M2,Integrin - β1,and L - PK,and immunofluorescence was used to detect the expression of Integrin- β1. A repeated measures analysis of variance was used for comparison of continuous data between groups. Results The CD34 ＋ cells inthe medium containing the serum collected after plasma exchange grew better than those in the medium containing the serum collected beforeplasma exchange （ P 〈 0. 05） . PK - M2 and Integrin - β1 were detected in the CD34 ^＋ cells of both groups,but L - PK was not detected ineither group. Conclusion For patients with liver failure,the improvement in internal environment after plasma exchange helps to maintainthe bioactivity of peripheral blood CD34^ ＋ cells,thus improving the efficacy of stem cell transplantation for liver failure.