摘要
目的比较人与Balb/c小鼠口腔癌形成及颌下淋巴结转移过程中E-钙黏蛋白(E-cadherin)的表达情况。方法采用免疫组化法检测人(24例)和小鼠(12例)正常口腔黏膜组织、口腔上皮异常增生组织、口腔鳞癌组织及口腔鳞癌颌下淋巴结转移灶中E-cadherin的表达情况。结果人正常口腔黏膜E-cadherin的表达率为100. 0%(24/24),口腔上皮异常增生组织E-cadherin表达率为87. 5%(21/24),口腔鳞癌E-cadherin表达率为0. 0%(0/24),颌下淋巴结转移灶中E-cadherin表达率为79. 2%(19/24)。小鼠正常口腔黏膜E-cadherin的表达率为100. 0%(12/12),口腔上皮异常增生组织E-cadherin表达率为83. 3%(10/12),口腔鳞癌E-cadherin表达率为0. 0%(0/12),颌下淋巴结转移灶中E-cadherin表达率为100. 0%(12/12)。结论 E-cadherin的表达在人与小鼠具有相似性,随着病理恶性程度的升高,人和小鼠口腔癌各阶段组织中E-cadherin的表达率逐渐降低,但在颌下淋巴结转移灶中表达率升高,表明E-cadherin是参与口腔癌发生、发展及颌下淋巴结转移过程中的一个重要因子。
Objective To compare the expressions of E-cadherin in different oral carcinogenesis stages and lymph node metastasis between human and mouse models. Methods Immunohistochemical staining was used to detect the expressions of E-cadherin in normal oral mucosae, oral dysplasia, oral squamous cell carcinomas(OSCC) and lymph node metastases in human beings( n =24) and mice( n =12), respectively. Results The expression rates of E-cadherin in normal oral mucosa, dysplasia, OSCC and lymph node metastasis were 100.0%, 87.5%, 0.0% and 79.2% in human beings and 100.0%, 83.3%, 0.0% and 100.0% in mice. Conclusion The expressions of E-cadherin are similar in the same pathological stages of human beings and mice respectively. The expression rates of E-cadherin are decreased gradually in both human beings and mice with the increased degree of pathological malignancy, but are increased in lymph node metastases. These results indicate that E-cadherin may play an important role in the occurrence and development of OSCC and submandibular lymphatic node metastase.
作者
程立
李晶
郭梦竹
牟云
陈念
于大海
CHENG Li;LI Jing;GUO Meng-zhu(Department of Oral and Maxillofacial Surgery,the Affiliated Stomatological Hospital of Guangxi Medical University,Nanning 530021,China)
出处
《中国临床新医学》
2018年第11期1094-1097,共4页
CHINESE JOURNAL OF NEW CLINICAL MEDICINE
基金
国家自然科学基金资助项目(编号:81360407)
广西自然科学基金资助项目(编号:2013GXNSFAA019182)
关键词
E-钙黏蛋白
口腔鳞状细胞癌
淋巴结转移
小鼠
人
E-cadherin
Oral squamous cell carcinoma(OSCC)
Lymph node metastasis
Mouse
Human