马鞭草中多酚类物质的提取及抗氧化活性检测

Extraction and Antioxidation of Polysaccharides from Verbena

本研究以马鞭草为原料,对多酚类物质的提取条件及抗氧化活性进行研究。研究结果显示:对马鞭草中多酚类物质提取率影响最显著的是提取温度,其次为提取时间,最后是提取溶剂。马鞭草中提取率最高的提取条件为:提取温度60℃,提取时间5 h、溶剂采用40%乙醇,此时多酚提取率达到0.402 4 mg/g。在最佳提取条件下获取的粗提液的DPPH清除率为25.74%。

In this study, we researched the extraction conditions and antioxidant activities of polyphenols from Verbena. The results showed that the extraction temperature, extraction time and extraction solvent had the three most significant effect on the extraction rate of polyphenols from Verbena. The extraction conditions of Verbena with the highest extraction rate were that the extraction temperature was 60 ℃, the extraction time was 5 h, and the extraction solvent was 40% ethanol solution. Under the optimum extraction conditions the DPPH scavenging rate was 48.74%.