PXDN表达水平对乳腺癌患者术后远期生存及预后的价值

Expression of PXDN in patients with breast cancer and its clinical value for the prognosis

目的:探讨乳腺癌组织过氧蛋白(PXDN)表达及其对预后的价值。方法:采用定量逆转录聚合酶链反应(qRT-PCR)和免疫组织化学染色检测55例乳腺癌组织和30例良性结节PXDN表达,Kaplan-Meier法绘制不同PXDN表达乳腺癌患者总体生存和无瘤生存曲线,Cox多因素回归模型分析乳腺癌预后的独立危险因素。结果:乳腺癌组织PXDN mRNA相对表达水平和PXDN染色评分明显高于良性结节组织,差异有统计学意义(P<0.05)。乳腺癌组织PXDN表达与年龄、肿瘤大小、TNM分期、人表皮生长因子受体2(Her-2)表达情况无关(P>0.05);PXDN表达与淋巴结转移、雌激素受体(ER)阴性和孕激素受体(PR)阴性有关(均P<0.05)。PXDN高表达组中位无瘤生存时间为53个月,PXDN低表达组中位无瘤生存时间为61个月,差异有统计学意义(χ~2=4.185,P=0.041)。PXDN高表达组术后中位总体生存时间为64个月,PXDN低表达组中位总体生存时间为73个月,差异有统计学意义(χ~2=4.315,P=0.038)。ER、PR、Her-2是影响乳腺癌无瘤生存的有意义风险因素(均P<0.05);ER、PR、Her-2和PXDN表达是影响乳腺癌总体生存的有意义风险因素(均P<0.05)。结论:乳腺癌组织PXDN表达上调,PXDN高表达是影响乳腺癌患者总体生存时间的独立危险因素。

Objective：To explore the expression of PXDN in breast cancer and its clinical value for the prognostic prediction.Method：Expression of PXDN in fifty-five breast cancer tissues and thirty benign nodule tissues were detected by using reverse transcription-polymerase chain reaction and immunohistochemical staining,respectively.The over-all survival and disease-free survival curve was drawn by Kaplan-Meier method in different PXDN expression group,independent prognostic factors were analyzed by Cox proportional hazards model.Result：The expression leves and ICH scores of PXDN were increased in the breast cancer tissues when compared to the benign nodule tissues,there was a significant difference between the two groups（P〈0.05）.PXDN expression was closely correlated with lymph node metastasis,negtive status of ER and PR（P〈0.05）,but not with age,tumor size,TNM stage,Her-2 status（P〉0.05）.The median disease-free survival time of the high PXDN group were 53 months,and61 months in the low PXDN group,there was a significant difference between the two groups（χ^2=4.185,P=0.041）.The median over-all survival time of the high PXDN group were 64 months,and 73 months in the low PXDN group,there was a significant difference between the two groups（χ^2=4.315,P=0.038）.Cox multivariate regression analysis showed that ER,PR,Her-2 status was independently associated with disease-free survival of breast cancer,wheras ER,PR,Her-2 status and PXDN expression was independently associated with over-all survival of breast cancer（all P〈0.05）.Conclusion：PXDN is upregulated in breast cancer.PXDN is an independent prognostic indicator of over-all survival time in patients with breast cancer.