数字PCR在功能核酸精准检测中的研究进展

Development Progress of Digital PCR in the Precise Detection of Functional Nucleic Acid

数字PCR是近年来迅速发展起来的一种定量分析技术。该技术结果判定不依赖于扩增曲线的循环阈值(Ct),不受扩增效率的影响,具有很好的准确度和重现性,并且可以实现绝对定量分析。数字PCR已经在功能核酸检测、鉴定等研究领域显示出巨大的技术优势和应用前景。在对数字PCR技术的基本原理和定量方法介绍的基础上,对该技术在功能核酸检测的主要应用领域进行综述,并对数字PCR在功能核酸检测领域中的研究前景做出了展望。

Digital PCR,as a quantitative analysis technology,has developed rapidly in recent years. The analysis of results from it doesn＇t depend on the cycle of threshold（Ct）of the amplification curve and is also not affected by the amplification efficiency,it has avery promising accuracy and reproducibility,and the absolute quantitative analysis can be achieved. Digital PCR has shown great advantagesand application prospects in the detection and identification of functional nucleic acids. Based on the introduction of the basic principles andquantitative methods of digital PCR technology,this paper reviews the main application fields and forecasts the research prospect of digital PCR in the detection of functional nucleic acids.