海鞘真菌的形态鉴定及其代谢产物抗菌活性研究

Morphological Identification and Antibacterial Activity on Metabolites of Fungi Isolated from Ascidean

为寻找产生新颖抗菌活性物质的海洋真菌,以海鞘为原料,采用平板涂布法分离出14株真菌,并对其进行形态学鉴定和抗菌活性的初筛。结果显示:从海鞘中共分离出14株真菌,根据形态观察初步鉴定其分属青霉属(Penicillium sp.)、曲霉属(Aspergillus sp.)、枝孢属(Cladosporium sp.)、麦轴梗霉属(Tritirachium sp.)等4个属。14株菌中有抗菌活性的共11株,优选出抗菌活性较强抗菌谱较广的4株菌分别是Asc-2-4(青霉属)、Asc-2-12(青霉属)、Asc-1-1(青霉属)和Asc-2-1(曲霉属)。青霉属菌株Asc-2-12的抑菌活性最明显,抑菌圈直径最大达到21.91 mm。海鞘共附生真菌是寻找抗菌物质的一个资源,其中的3株青霉属和1株曲霉属的抗菌活性有深入研究的价值。

To seek new species of marine fungi with high antibacterial activity,ascidean was used as study material,and 14 fungi were isolated by plate spreading method,and their morphological identification and preliminary screening of their antibacterial activity wasconducted. The results indicated that 14 fungal strains were isolated from ascidian,and preliminarily identified as Penicillium sp.,Aspergillus sp.,Cladosporium sp.,and Tritirachium sp. based on the morphological observation. Total 11 fungal strains of 14 presented antibacterialactivity,and 4 strains of Asc-2-4（Penicillium sp.）,Asc-2-12（Penicillium sp.）,Asc-1-1（Penicillium sp.）,and Asc-2-1（Aspergillus sp.）had broad-spectrum and strong antibacterial activity. The antibacterial activity of Asc-2-12（Penicillium sp.）was the most obvious,withthe diameter of maximum inhibition circle reaching 21.91 mm. Symbiotic fungal strains from ascidian are potential antibacterial resources and Asc-2-4,Asc-2-12,Asc-1-1,and Asc-2-1 have potential prospect to be further investigated.