脂肪酶对邻苯二甲酸二甲酯的催化降解性能研究

Study on Catalytic Degradation Performance of Dimethyl Phthalate by Lipase

采用脂肪酶(GZ-1)、α-淀粉酶(AD-1)和胃蛋白酶(GW-1)作为催化剂,研究其对水中邻苯二甲酸二甲酯(DMP)的降解性能。结果表明,GZ-1、AD-1和GW-1对DMP降解的适宜pH分别约为7.0、6.7和5.4。在277～318K时,3种酶对DMP的去除率均在308 K达到最大,且GZ-1对DMP的去除率为83.2%,明显高于AD-1(76.1%)和GW-1(65.4%)。在288～318 K时,GZ-1对DMP的去除在60 min左右达到平衡,去除速率随温度的升高先增大后减小,在308 K时达到最大;质量浓度为40 mg/L的GZ-1对DMP的去除速率最快。与蒸馏水相比,自来水中物质对GZ-1的催化性能影响不大,GZ-1可以作为高效催化剂快速降解水中DMP。

The catalytic degradation performance of dimethyl phthalate （DMP） in water by three enzymes lipase （GZ-1 ）, a-amylase （AD-1） and pepsin （GW- 1） were investigated. The results showed that the optimum pH for the degradation of DMP by GZ-1, AD-1 and GW-1 were 7.0, 6.7 and 5.4, respectively. In the temperature range of277-318 K, the removal efflciencies of DMP all reached the maximum at 308K for the three enzymes. Moreover, the removal efficiency of DMP by GZ-1 was 83.2%, which was significantly higher than that ofAD-1 （76.1%） and GW-1 （65.4%）. That in the temperature range of 288-318 K, the removal equilibrium of DMP by GZ-1 reached at around 60 min, and the removal rate Increased first and then decreased with the increase of temperature, and reached the maximum at 308 K. When the mass concentration of GZ-1 was 40 mg/L, the removal rate reached the maximum. Compared to the distilled water, little difference was found in the catalytic degradation of DMP in tap water. Therefore, GZ-1 could be used as an efficient catalyst to rapidly remove DMP in water.