产地加工炮制一体化香薷的解热抗炎作用研究

Antipyretic and anti-inflammatory effects of Mosla chinensis based on integration processing technology of origin

目的比较产地加工炮制一体化制备香薷与传统切制香薷对脂多糖(LPS)致热大鼠和LPS刺激的小鼠单核巨噬细胞RAW264.7炎症的作用,为香薷产地加工炮制一体化技术实施及香薷辛温解表的传统功效提供科学依据。方法以香薷(挥发油和水煎液)进行药物干预后,采用ip LPS造大鼠发热模型,观察发热大鼠体温变化情况,检测大鼠血清前列腺素E2(PGE2)、肿瘤坏死因子-α(TNF-α)、环磷酸腺苷(c AMP)、白细胞介素-1β(IL-1β)和肝组织髓过氧化物酶(MPO)水平,比较产地加工炮制一体化和传统切制香薷对热证大鼠的影响;以不同浓度香薷(挥发油和水煎液)作用于LPS刺激的RAW264.7细胞,测定细胞存活率,ELISA法检测NO分泌量,比较产地加工炮制一体化和传统切制香薷的抗炎作用。结果各给药组能够降低大鼠肛温及PGE2、TNF-α、c AMP、IL-1β、MPO含量,增强细胞免疫作用,且产地加工炮制一体化组比传统切制组作用显著,香薷水煎液作用略优于挥发油。结论香薷产地加工炮制一体化技术可行。

Objective To compare the effect of integration processing technology of origin with traditional cutting processing technology of Mosla chinensis on pyretic rats caused by LPS and inflammatory RAW264.7 cells induced by LPS,and provide the feasible basis for the implementation of integration processing technology of origin and traditional efficacy of the relieving exterior effects of M.chinensis.Methods The rats were intervened by M.chinensis（volatile oil,decoction）,and the model of heat syndrome was established by ip of LPS.The temperature changes of the fever rats were observed.The levels of serum prostaglandin E2（PGE2）,tumor necrosis factor-α（TNF-α）,adenosine cyclic phosphate（c AMP）,interleukin-1β（IL-1β）,and myeloperoxidase（MPO） in liver tissue were detected.The effects of integration processing of origin and traditional cutting processing of M.chinensis on heat syndrome rats were compared.The cell viability of LPS stimulated RAW264.7 macrophages cells after administration of different concentration of M.chinensis（decoction and volatile oil） was detected,and the NO secretion was determinated by ELISA for comparing the anti-inflammatory effect of two processing methods.Results The treatment of processed M.chinensis can decrease the anus temperature and the content of PGE2,TNF-α,CAMP,IL-1β,MPO,and enhance cellular immunity.Moreover the efficacy of integration processing of origin is better than traditional cutting processing,and the efficacy of M.chinensis decoction is better than volatile oil.Conclusion The integration processing technology of origin for M.chinensis is feasible.