雷公山国家级自然保护区7种棉革菌外生菌根形态描述和分子鉴定

Morphological Description and Molecular Identification of Seven Tomentella Ectomycorrhizae from Leigong Mountain Nature Reserve

[目的]研究雷公山国家级自然保护区的棉革菌属外生菌根的分布情况,探索外生菌根与林木互利共生的关系。[方法]采用表型分析和r DNA ITS序列分析等方法,对贵州雷公山国家级自然保护区的常绿阔叶林、落叶阔叶林、暖性针叶林、灌丛4种优势林分的外生菌根进行研究。[结果]表明:基于形态及解剖结构特征分析,从4种优势林分中分离得到7种具有棉革菌属(Tomentella)典型特征(如颜色为棕色)的外生菌根; r DNA ITS区段系统发育分析表明,分离得到的7种外生菌根隶属棉革菌属,并可将其中6种形态型分别鉴定为T. badia、T. fuscocinerea、T.atramentaria、T. lapida、T. ramosissima和T. terrestris。[结论]首次从雷公山国家级自然保护区发现棉革菌属外生菌根真菌6种,为该属外生菌根与优势林分的共生关系及功能研究等提供参考。

[Objective]To explore the ectomycorrhizae types and relationship with forest stands at Leigong Mountain Nature Reserve,and to study the ectomycorrhizae in four preponderant forest stands. [Method]The methods of random sampling and r DNA ITS sequencing were applied in this study. [Result]The results showed that seven types of genus Tomentella were determined by phenotype with brown morphotypes that was similar with genus Tomentella,and the results was verified by r DNA ITS sequencing,. Six of them were identified as T. badia,T. fuscocinerea,T. atramentaria,T. lapida,T. ramosissima and T. terrestris,respectively. [Conclusion] This research is the first report that the genus Tomentella was found at Leigong Mountain Nature Reserve,so the results will benefit for next research at commensalism and function with four preponderant forest stands at Leigong Mountain Nature Reserve.