基于丹参药材内在质量与外观色泽相关性分析探索传统鉴定经验“色红者佳”的科学性

Scientific Analysis of Traditional Identification Experience of “Redder is Better” Based on Correlation Between Intrinsic Quality and Appearance Color of Salviae Miltiorrhizae Radix et Rhizoma

目的：探究丹参药材指标成分及浸出物含量与外观色泽的相关性。方法：采用HPLC对18批丹参药材中有效成分（丹酚酸B和丹参酮类成分）的含量进行测定,按2015年版《中国药典》收录的方法对丹参药材的浸出物进行测定,使用色差仪测定丹参粉末甲醇溶液的色度值,在此基础上,分别对有效成分、浸出物含量和色度进行相关性研究。结果：丹参中有效成分含量越高,浸出物含量越高,a＊（红绿分量值）越大,外观红色越深,药材质量越好;有效成分含量越低,浸出物含量越低,a＊越小,外观红色越浅,药材质量越差。结论：通过丹参内在质量与外观质量关联度分析,确立了一种可用于判断丹参质量的简便方法,验证了传统鉴别经验＂色红者佳＂的科学性。

Objective： To investigate the correlation between intrinsic quality and the appearance color of Salviae Miltiorrhizae Radix et Rhizoma. Method： The contents of effective ingredients（salvianolic acid B and tanshinones） in Salviae Miltiorrhizae Radix et Rhizoma were determined by HPLC. The extract content was determined by methods in the 2015 edition of Chinese Pharmacopoeia and the chromatic values of methanol solution of Salviae Miltiorrhizae Radix et Rhizoma powder were measured by colorimeter. On the basis of this,the three sets of data were fitted to find out the relationships. Result： The contents of effective ingredients were higer,the content of extract was higher,the value of a＊（red and green weight value） was higher,and the color was redder,then the quality of Salviae Miltiorrhizae Radix et Rhizoma was better; the opposite is the same.Conclusion： A simple method,which is used to judge the quality of Salviae Miltiorrhizae Radix et Rhizoma,is established based on the correlation analysis of appearance and intrinsic quality,this method can be also used to verify scientificity of traditional identification experience of ＂redder is better＂.