白芍饮片炒制前后物质基础的变化规律分析

Analysis on Change Law of Substance Basis in Paeoniae Radix Alba Decoction Pieces Before and After Being Processed

目的：基于HPLC特征图谱和主要成分的含量测定探讨白芍炒制前后饮片物质基础的变化规律。方法：采用RP-HPLC,选择ZORBAX SB-C18色谱柱（4.6 mm×250 mm,5μm）,乙腈（A）-0.05%磷酸水溶液（B）为流动相梯度洗脱（0~5 min,5%~9%A;5~25 min,9%~17%A;25~30 min,17%~22%A;30~35 min,22%A;35~50 min,22%~40%A;50~60 min,40%A）,流速1.0 m L·min-1,柱温30℃,进样量10μL,检测波长254 nm,建立白芍及炒白芍饮片特征图谱表征方法;同时对没食子酸等6种成分进行定量分析（检测波长230 nm）。以乙腈-0.05%磷酸水溶液（18∶82）为流动相等度洗脱,同时测定1,2,3,4,6-五没食子酰基葡萄糖与苯甲酸的含量。结果：白芍与炒白芍饮片特征图谱中均标示出12个特征峰,并归属了其中8个特征峰。白芍炒制后单萜苷类成分芍药苷与芍药内酯苷质量分数分别降低了6%和7%,鞣质类成分含量显著增加,没食子酸和1,2,3,4,6-五没食子酰基葡萄糖、苯甲酸的质量分数分别增加了28%,26%,53%,其余成分的含量均无明显变化。结论：白芍炒制前后特征图谱及主要成分含量均有一定差异,本文所建立的特征图谱及含量测定方法为白芍和炒白芍饮片质量评价标准的制定提供了参考,为进一步完善二者的质量评价体系奠定了基础。

Objective： Based on the HPLC specific chromatograms and the contents of main chemical components,to discuss the change law of substance basis in Paeoniae Radix Alba decoction pieces before and after being processed. Method： RP-HPLC was employed with ZORBAX SB-C18 column（4. 6 mm × 250 mm,5 μm）,a gradient mobile phase of acetonitrile-0. 05% phosphoric acid aqueous solution was eluted, the column temperature was 30 ℃ and the flow rate was 1. 0 m L·min-1,detection wavelength was set at 254 nm,the method for characterizing the characteristics of Paeoniae Radix Alba decoction pieces was established; at the same time,gallic acid and other 5 ingredients were simultaneously determined at 230 nm. The contents of 1,2,3,4,6-pentagalloylglucose and benzoic acid were simultaneously determined with mobile phase of acetonitrile-0. 05%phosphoric acid aqueous solution（18 ∶ 82）. Result： Paeoniae Radix Alba and fired Paeoniae Radix Alba decoction pieces were marked with twelve characteristic peaks, and eight of them were assigned. After being processed,the contents of paeoniflorin and albiflorin decreased by 6% and 7%, respectively; the content of tannins increased significantly,the contents of gallic acid,1,2,3,4,6-pentagalloylglucose and benzoic acid increased by 28%, 26% and 53%, respectively; while the contents of other components did not change significantly. Conclusion： According to the specific chromatograms and contents of main chemical components in this article,it can provide reference for formulation of quality evaluation criteria of Paeoniae Radix Alba and fired Paeoniae Radix Alba decoction pieces,which lays the foundation for further improving the quality evaluation system of them.