摘要
目的:研究小金丹对乳腺癌细胞侵袭和转移的影响及其作用机制。方法:选择高转移性乳腺癌细胞株MDA-MB-453细胞株,用不同浓度的小金丹提取物(25、50、100、150、200mg生药/L)处理细胞24h后,分别采用肿瘤细胞黏附实验、划痕实验和Transwell侵袭实验检测细胞黏附、运动、侵袭能力;Western blot法检测上皮-间质转化(EMT)相关蛋白和丝裂原活化蛋白激酶(MAPK)通路蛋白的表达水平。结果:与对照组比较,小金丹提取物作用24h后能明显抑制人乳腺癌MDA-MB-453细胞的运动和侵袭能力(P<0.01),作用呈现剂量依赖性关系(P<0.01);与对照组比较,100、150mg/L小金丹提取物能明显增加ZO-1和β-catenin表达(P<0.01),降低Vimentin表达(P<0.01),明显抑制p-JNK和p-p38的蛋白表达水平(P<0.01)。结论:小金丹能有效地抑制高转移性乳腺癌细胞的运动和侵袭能力,作用机制可能与其调控p38 MAPK和JNK MAPK通路,逆转肿瘤细胞EMT有关。
Objective: To study the effect of Xiaojindan(XJD) on invasion and metastasis of breast cancer cells and its mechanism. Methods: High-metastatic breast cancer cell line MDA-MB-453 was selected. Cells were treated with different concentrations of XJD extract(25, 50, 100, 150, 200 mg crude drug/L) for 24 h, respectively. The cell adhesion, movement and invasion ability were detected by Tumor cell adhesionassay, scratchassay and Transwell invasion assay. The expression levels of epithelial-mesenchymal transition(EMT)-associated protein and mitogen-activated protein kinase(MAPK) pathway protein were detected by Western blot. Results: Compared with the control group, XJD could significantly inhibit the movement and invasion of breast cancer MDA-MB-453 cells in a dose-dependent manner after 24 hours(P0.01). Compared with the control group, XJD extract 100, 150 mg/L could increase the expression of ZO-1 and β-catenin(P0.01), and the expression of Vimentin was decreased(P0.01), significantly inhibit the protein expression levels of p-JNK and p-p38(P0.01). Conclusion: XJD can effectively inhibit the migration and invasion of high metastatic breast cancer cells. The mechanism may be related to the regulation of p38 MAPK and JNK MAPK pathway and the reversal of tumor cell EMT.
作者
彭博
贺蓉
徐启华
孙丽华
韩靖雅
李建荣
PENG Bo;HE Rong;XU Qi-hua;SUN Li-hua;HAN Jing-ya;LI Jian-rong(lnstitute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100029,China;Experimental Research Center,China Academy of Chinese Medical Sciences,Beijing 100700,China)
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2018年第11期4916-4919,共4页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金项目(No.81302981)
中央级公益性科研院所基本科研业务费专项资金项目(No.ZXKT17023
No.ZXKT17051
No.ZZ2015002)~~
关键词
乳腺癌
小金丹
肿瘤转移
MAPK通路
上皮间质转化
Breast cancer
Xiaojindan
Metastasis
MAPK pathway
Epithelial-mesenchymal transition