人参总蛋白对帕金森病转基因果蝇模型的脑神经保护作用研究

Neuroprotective effect of total protein of Panax ginseng on Drosophila melanogaster model with Parkinson's disease

目的探讨人参总蛋白对帕金森病(PD)模型果蝇脑神经的保护作用及其可能机制。方法将α-突触核蛋白异常表达的果蝇(PD模型果蝇)分别饲养于正常培养基以及混合0.02、0.04、0.16 g/L人参总蛋白的含药培养基中,研究人参总蛋白对PD模型果蝇的生存状态、寿命、爬行能力及氧化应激水平的影响。采用TBARS法检测果蝇脑部脂质过氧化物(LPO)含量,DTNB法测定谷胱甘肽(GSH)含量,CDNB法检测谷胱甘肽-S-转移酶(GST)活力,DNPH法检测蛋白质羰基(PC)含量。结果人参总蛋白能够改善PD模型果蝇存活率降低以及爬行能力异常的状态,并存在一定的浓度依赖性;与对照组比较,PD模型果蝇脑部LPO、GST、PC含量升高,GSH含量降低;人参总蛋白可显著降低PD模型果蝇脑部LPO(P<0.01)、GST(P<0.000 1)、PC(P<0.000 1)含量,升高GSH(P<0.000 1)含量。结论人参总蛋白能有效降低PD模型果蝇的病理症状,该作用可能是通过降低氧化应激压力实现的。

Objective To investigate the neuroprotective effect of ginseng total protein（GTP） on Parkinson＇s disease（PD） Drosophila melanogaster model and its possible mechanism. Methods D. melanogaster with abnormal expression of α-synuclein was cultured in normal medium and mixed with 0.02, 0.04, and 0.16 g/L of GTP in the drug-containing medium to study the effects of GTP on the survival, lifespan, crawling ability, and oxidative stress of PD model D. melanogaster. TBARS method was used to detect the content of LPO, DTNB method was used to determine GSH content, CDNB method was used to detect GST activity, and DNPH method was used to detect protein carbonyl（PC） content. Results GTP can improve the decrease of D. melanogasterlivability and abnormal crawling ability caused by α-synuclein mutation in a dose dependent manner. The content of LPO, GST, and PC in D. melanogaster with α-synuclein mutation were increased, and the content of GSH was decreased. The GTP significantly reduced the contents of LPO（P 0.01）, GST（P 0.000 1）, and PC（P 0.000 1）, and significantly increased GSH（P 0.000 1） content. Conclusion GTP can effectively reduce the pathological symptoms of PD drosophila model. Its effect may be achieved by reducing the oxidative stress.