摘要
Burmanniaceae is one major group within the monocot order Dioscoreales that has not had its plastome sequenced.Members of Burmanniaceae are mostly achlorophyllous,although the genus Burmannia also includes autotrophs.Here,we report sequencing and analysis of the first Burmanniaceae plastid genome from Burmannia disticha L..This plastome is 157,480 bp and was assembled as a circular sequence with the typical quadripartite structure of plant plastid genomes.This plastome has a regular number of potentially functional genes with a total of 111,including 78 protein coding genes,4 ribosomal RNA(rRNA) genes,and 29 tRNA genes.The ratio of the total length of genic:intergenic DNA is 1.58:1,and the mean length of intergenic regions is 398 bp,the longest being 1918 bp.The overall GC content of the B.disticha plastome is 34.90%,and the IR regions in B.disticha are more GC rich(39.50%) than the LSC(32.30%) and SSC(28.80%) regions.Phylogenetic analysis of protein-coding sequences from plastomes of related species in the order Dioscoreales support a clade comprising Burmanniaceae and Dioscoreaceae.This phylogenetic placement is congruent with previous findings based on nuclear and mitochondrial evidence.
Burmanniaceae is one major group within the monocot order Dioscoreales that has not had its plastome sequenced. Members of Burmanniaceae are mostly achlorophyllous, although the genus Burmannia also includes autotrophs. Here, we report sequencing and analysis of the first Burmanniaceae plastid genome from Burmannia disticha L.. This plastome is 157,480 bp and was assembled as a circular sequence with the typical quadripartite structure of plant plastid genomes. This plastome has a regular number of potentially functional genes with a total of 111, including 78 protein coding genes, 4 ribosomal RNA （rRNA） genes, and 29 tRNA genes. The ratio of the total length of genic：intergenic DNA is 1.58：1, and the mean length of intergenic regions is 398 bp, the longest being 1918 bp. The overall GC content of the B. disticha plastome is 34.90% and the IR regions in B. disticha are more GC rich （39.50%） than the LSC （32.30%） and SSC （28.80%） regions. Phylogenetic analysis of protein-coding sequences from plastomes of related species in the order Dioscoreales support a clade comprising Burmanniaceae and Dioscoreaceae. This phylogenetic placement is congruent with previous findings based on nuclear and mitochondrial evidence.
基金
supported by the Large-scale Scientific Facilities of the Chinese Academy of Sciences(Grant No:2017-LSFGBOWS-02)
the Youth Innovation Promotion Association of Chinese Academy of Sciences(2015321)
关键词
自养生物
生物学
植物
栀子科
Burrnannia disticha
Burmanniaceae
Plastome
Phylogenetic analysis
