GeneXpert MTB/RIF检测细针穿刺活检组织洗脱液对肺外结核的辅助诊断价值

Auxiliary diagnostic value of GeneXpert MTB/RIF assay for extrapulmonary tuberculosis of fine needle biopsy tissue eluent

为评价GeneXpert MTB/RIF（简称“GeneXpert”）检测细针穿刺活检组织洗脱液对肺外结核的辅助诊断价值,笔者分别采用荧光定量PCR（TB-DNA PCR）、GeneXpert、固体培养法及涂片法检测武汉市肺科医院2016年1月至2017年12月期间确诊的103例肺外结核患者病变部位细针穿刺活检组织洗脱液。结果显示,GeneXpert检测敏感度[78.6%（81/103）]明显高于固体培养法[26.2%（27/103）]和涂片法[16.5%（17/103）],差异均有统计学意义（χ 2值分别为56.76和79.72,P值均〈0.05）。可见,GeneXpert检测细针穿刺活检组织洗脱液对肺外结核的诊断敏感度较高。

To evaluate the auxiliary diagnostic value of GeneXpert MTB/RIF （GeneXpert） for detecting extrapulmonary tuberculosis based on fine needle biopsy tissue eluent, a total of 103 patients with extrapulmonary tuberculosis in Wuhan Pulmonary Hospital between January 2016 and December 2017 were enrolled. All of these patients were operated through fine needle puncture and the biopsy tissue eluent were tested by tuberculosis-DNA PCR assay, GeneXpert, tuberculosis solid culture and smear method, respectively. The results showed that the sensitivity of GeneXpert （78.6% （81/103）） was significantly higher than that of tuberculosis solid culture （26.2% （27/103）） and smear method （16.5% （17/103））, and the differences were statistically significant （χ 2 values were 56.76 and 79.72, respectively; all P values 〈0.05）. Hence, it is clear that GeneXpert showed a higher diagnostic sensitivity for detecting extrapulmonary tuberculosis based on fine needle biopsy tissue eluent.