维生素C对大豆胰蛋白酶抑制因子诱导氧化应激的干预

Vitamin C against oxidative stress induced by soybean trypsin inhibitor

旨在研究不同剂量的维生素C（Vc）对大豆胰蛋白酶抑制因子（STI）诱导的小鼠胰腺氧化应激的干预作用。试验分为4个处理组,每组12只清洁级雄性小鼠,第1组为正常对照组,饲喂基础日粮;第2组为模型组（STI组）,饲喂添加STI的日粮;第3组为Vc1组,饲喂添加STI和低剂量Vc的日粮;第4组为Vc2组,饲喂添加STI和高剂量Vc的日粮。3周后处死小鼠,测定其氧化及抗氧化指标的变化。试验结果所示,STI组胰腺和血清中的MDA含量与对照组相比显著升高（P〈0.05）,总超氧化物歧化酶（T-SOD）、过氧化氢酶（CAT）等酶活性与对照组相比显著降低（P〈0.05）。低剂量Vc组胰腺和血清中的MDA含量与STI组相比显著降低（P〈0.05）,CAT、T-SOD等活性与STI组相比显著升高（P〈0.05）。高剂量Vc组胰腺和血清中MDA含量与低剂量组相比显著升高（P〈0.05）,CAT、T-SOD等活性与低剂量组相比显著降低（P〈0.05）。本研究表明,适当剂量的Vc可以降低STI诱导的小鼠胰腺氧化应激程度,起到抗氧化作用,但其抗氧化能力与添加剂量密切相关,过量摄入Vc反而会促进氧化。

The current study was performed to assess the influence of vitamin C under different do- ses against oxidative stress induced by soybean trypsin inhibitor （STI） in pancreas of mice. 48 male KM mice were divided into four groups. Control group received normal diets;STI group re- ceived normal diets containing STI, Vcl and Vc2 group received STI diets containing 2 mg/g, 5 mg/g vitamin C respectively. After 3 weeks, the mice were sacrificed. The results showed that compared with control group,MDA contents in serum and pancreas of STI group were significant- ly increased （P〈0.05）,the activities of CAT, T-SOD and other enzymes were significantly de- creased （P〈0.05）. Compared with STI group. MDA contents in serum and pancreas of Vcl group were significantly decreased （P〈0.05） ,the activities of CAT,T-SOD and other enzymes were sig- nificantly increased （P〈0.05）. Compared with Vcl group, MDA contents in serum and pancreas of Vc2 group were significantly increased （P〈0.05）, the activities of CAT, T-SOD and other en- zymes were significantly decreased （P〈0.05）. The present study indicates that vitamin C can de- crease the effect of soybean trypsin inhibitor oxidative stress. But the degree of antioxidant capacity is closely related with dose, excessive intake of this vitamin can facilitate oxidation.