载脂蛋白A5蛋白在胆管癌组织的表达及对胆管癌细胞生物学特性的影响

Expression of apolipoprotein A5 in cholangiocarcinoma and its effect on biological characteristics of cholangiocarcinoma cells

目的探讨胆管癌组织载脂蛋白A5（APOA5）蛋白的表达及对胆管癌细胞生物学特性的影响。方法收集胆管癌组织和癌旁组织，采用免疫组织化学分析APOA5蛋白的表达水平。采用慢病毒感染胆管癌细胞QBC939建立对照细胞株和APOA5敲低稳定细胞株，采用细胞计数试剂盒（CCK-8）测定细胞增殖，采用Transwell技术分析胆管癌细胞迁移能力，采用裸鼠体内成瘤实验分析两组细胞在体内成瘤。结果与癌旁组织APOA5蛋白表达水平（129.43±20.43）比较，胆管癌组织中APOA5蛋白表达水平（494.28±31.87）显著上调，差异有统计学意义（t=4.912，P=0.000）。与对照组细胞比较，APOA5短发卡RNA（shRNA）组细胞的增殖能力明显下降，差异有统计学意义（F=3.901，P=0.000）。与对照组[（421.25±31.44）个]比较，APOA5 shRNA组细胞迁移能力[（138.33±23.76）个]显著下调，差异有统计学意义（t=5.109，P=0.000）。与对照组比较，APOA5 shRNA组小鼠肿瘤生长速度明显减缓，差异有统计学意义（F=2.918，P=0.014）。结论APOA5蛋白在胆管癌中表达水平显著升高，且与胆管癌细胞增殖、迁移和成瘤明显相关。

Objective To investigate the expression of apolipoprotein A5 （APOA5） protein in cholangiocarcinoma and its effect on the biological characteristics of cholangiocarcinoma cells. Methods Cholangioearcinoma tissues and adjacent tissues were collected and the expression level of APOA5 protein was analyzed by immunohistochemistry. The control cell line and APOA5 knockdown stable cell line were established in cholangioearcinoma cell line QBC939 by lentiviral. The proliferation of the cells was measured by cell counting kit-8 （CCK-8）. The migration ability of the bile duct cancer cells was analyzed by Transwell technique. The tumor formation of the two groups in the body was analyzed by Xenograft experiment in nude mice. Results Compared with the expression level of APOA5 protein （ 129.43±20. 43 ） in the paracancerous tissue, the expression of APOA5 protein （494. 28±31.87 ） in the bile duct carcinoma significantly increased （ t=4.912, P=0.000 ）. Compared with the control group, the proliferation of APOA5 short hairpin RNA （shRNA） cells decreased significantly （ F=3.901, P=0.000）. Compared with the control group [（421.25 ＋31.44） ], the cell migration ability of APOA5 shRNA group （ 138.33±23.76） significantly decreased （t=5. 109, P = 0.000）. Compared with the control group, the growth rate of tumor in APOA5 shRNA group slowed down significantly, the difference was statistically significant （F=2.918, P=0.014）. Conclusion The expression level of APOA5 protein in cholangiocarcinoma is significantly increased and is closely related to the proliferation, migration and tumor formation of cholaniocarcinoma cells.