HOXB7基因对裸鼠结肠癌肺转移模型的致癌机制研究

Carcinogenic mechanisms of HOXB7 in the nude mouse models of pulmonary metastases from colon cancer

目的探讨HOXB7基因对裸鼠结肠癌肺转移模型的致癌机制。方法将转染pcDNA6-scramble或pcDNA6-HOXB7基因重组质粒的HT29人结肠癌细胞株,经裸鼠尾静脉注入后建立稳定的结肠癌肺转移模型。模型裸鼠共20只,其中10只HOXB7组(研究组)和10只Scramble组(对照组)。制模8周,观察两组裸鼠肺转移癌情况及生存情况。结果两组制模成瘤率为100%(20/20)。研究组肿瘤体质量和肿瘤大小检测值分别为0.093 g和0.960 mm,明显大于对照组的0.069 g和0.720 mm,差异均有统计学意义(P<0.05);与对照组比较,研究组肿瘤多呈非边缘型、非类圆形、浸润型生长,差异均有统计学意义(P<0.05);两组生存曲线分析,发现研究组生存率为50%,明显劣于对照组的90%,差异有统计学意义(P<0.05)。结论 HOXB7基因可促使裸鼠结肠癌肺转移模型的转移形成,且可作为结肠癌肺转移的优良评价指标;HOXB7基因的加入可反馈结肠癌肺转移的模拟演变过程,且可被称为理想的实验小动物模型。

Objective To establish of carcinogenic mechanisms of HOXB7 in the nude mouse model of pulmonary metastases from colon cancer. Methods HT29 human colon cancer cells, transfected by pcDNA6-HOXB7 of recombinant vector or pcDNA6-scramble vector, were injected into tail vein of nude mouses to establish the nude mouse models of pulmonary metastases from colon cancer. Among the 20 cases who underwent pulmonary metastases from colon cancer model, 10 cases were transfected by recombinant vector pc DNA6-HOXB7（the study group） and 10 cases were transfected by pc DNA6-scramble vector（the control group）. The formation situation of metastatic tumor and the survival status of nude mouse models in the two groups were observed 8 weeks after the establishment of models. Results The rates of tumor formation were 100%（20/20） in the two groups. The tumor weight and tumor size of the study group were0.093 g and 0.960 mm, respectively, which were significantly greater than corresponding 0.069 g and 0.720 mm in the control group（P〈0.05）. Compared to the control group, the tumor in the study group were mostly non-marginal type,non-cycloidal type, and infiltrating type（P〈0.05）. The survival curves showed that the survival rate of the study group was 50%, which was significantly less than 90% in the control group（P〈0.05）. Conclusion HOXB7 gene may be promote the formation of the nude mouse model of pulmonary metastases from colon cancer, and it can be regarded as an indicator for evaluating pulmonary metastases from colon cancer. The addition of HOXB7 gene can feedback the simulated evolution of pulmonary metastases from colon cancer, and serve as an ideal experimental small animal model.