血清补体、免疫球蛋白及炎性细胞因子动态检测在儿童肺炎支原体感染中的应用价值分析

Analysis of the Application Value of Serum Complement, Immunoglobulin and Inflammatory Cytokines in the Detection of Mycoplasma Pneumoniae Infection in Children

目的研究儿童肺炎支原体感染中应用血清补体、免疫球蛋白及炎性细胞因子动态检测在的临床价值。方法方便选择该院2016年8月—2017年8月期间收治的100例儿童肺炎支原体感染患儿作为该次研究对象,作为研究组,并选取同期健康体检儿童100例作为对照组,应用免疫散射比浊法检测血清补体、免疫球蛋白,应用酶联免疫吸附法测定炎性细胞因子,对比两组的检测结果。结果研究组严重型IL-8、IL-13与TNF-α(123.45±48.24)、(243.52±46.21)、(98.29±9.12)pg/mL明显高于轻微型(72.42±40.21)、(154.21±23.97)、(63.11±8.00)pg/mL与对照组(28.45±6.52)、(80.51±10.31)、(40.54±2.31)g/L,严重型IgG、C3与C4(9.46±2.13)、(2.00±0.34)、(0.50±0.22)g/L显著高于轻微型(7.19±1.74)、(1.24±0.29)、(0.33±0.21)g/L与对照组(7.32±1.50)、(1.12±0.23)、(0.31±0.10)g/L,研究组急性期IL-10水平(115.58±34.16)pg/mL明显低于恢复期(55.41±11.57)pg/mL与对照组(28.34±6.50)pg/mL,组间对比差异有统计学意义(P<0.05)。结论儿童肺炎支原体感染感染有明显的细胞与体液免疫功能紊乱,利用血清补体、免疫球蛋白及炎性细胞因子动态检测能够有效判定疾病的分期与病情的程度。

Objective To study the clinical value of dynamic detection of serum complement, immunoglobulin and inflammatory cytokines in children with Mycoplasma pneumoniae infection. Methods 100 children with mycoplasma pneumoniae infection who were admitted to the hospital from August 2016 to August 2017 were convenient selected as the study group,and 100 healthy children in the same period were selected as the control group. Turbidity was used to detect serum complement and immunoglobulin, and inflammatory cytokines were determined by enzyme-linked immunosorbent assay. The results of the two groups were compared. Results The severe IL-8, IL-13 and TNF-α（123.45±48.24）,（243.52±46.21）,（98.29±9.12）pg/mL in the study group were significantly higher than the mild type（72.42±40.21）,（154.21±23.97）,（63.11±8.00）pg/mL and the control group（28.45±6.52）,（80.51±10.31）,（40.54±2.31）g/L, severe IgG, C3 and C4（9.46±2.13）,（2.00±0.34）,（0.50±0.22）g/L were significantly higher than mild（7.19±1.74）,（1.24±0.29）,（0.33±0.21）g/L and the control group（7.32±1.50）,（1.12±0.23）,（0.31±0.10）g/L, the IL-10 level in the acute phase of the study group（115.58±34.16）pg/mL was significantly lower than the recovery period（55.41±11.57）pg/mL and the control group（28.34±6.50）pg/mL, the statistical significance of the comparison between groups（P〈0.05）. Conclusion Children with Mycoplasma pneumoniae infection have obvious cell and humoral immune dysfunction. The dynamic detection of serum complement, immunoglobulin and inflammatory cytokines can effectively determine the stage and condition of the disease.