一种次氯酸根离子荧光探针的合成及荧光性能研究

Synthesis and Fluorescence Properties of a Novel Fluorescent Probe for Selective Detection of OCl^-

为通过荧光素肼与邻甲氧基苯甲醛反应合成得到目标探针.文中采用核磁共振(NMR)及高分辨质谱(HRMS)对探针的结构进行表征.通过荧光光谱仪测试探针对次氯酸根离子的荧光检测能力,包括:荧光滴定、荧光时间响应、离子干扰和pH响应等.结果表明:所合成的探针可排除17种相关离子的干扰实现对次氯酸根离子的检测,响应时间为8min,线性检测范围为y=45.16x-4.506,检测限为1μmol·L^(-1).表明该探针是一种高选择性、高灵敏度的荧光探针.

The probe which was synthesized through the reaction between fluorescein and o methoxybenzaldehyde was characterized by 1 H NMR, 13 C NMR spectra and mass data.Its sensing performance was studied by the fluorescence spectrophotometer,including fluorescence titration,time response,ion interference and pH influence experiments.The probe could eliminate the interference of 17 related ions,the response time was 8 minutes and the regression equation was y=45.16x-4.506 with a detection limit of 1 μmol·L ^-1 .The results show that the fluorescent probe is of high selectivity and sensitivity.