尼泊金丁酯用于24h尿液标本生化项目测定的研究

The effects of butylparaben used as a preservative on the biochemical determination of 24-hour urine specimen

目的研究尼泊金丁酯作为防腐剂对24h尿液标本生化项目测定结果的影响。方法收集新鲜无菌或有菌尿液标本各20份,每份分成4组。一组作为处理前原始标本组,定量检测尿液标本中微量总蛋白、葡萄糖、钾、钠、氯、钙、磷离子,以及肌酐、尿素的水平,同时进行细菌培养及菌落计数;其余3组为尼泊金丁酯组、甲苯组、无防腐剂组,均放置24h后定量检测上述指标。结果原始标本为无菌尿液时:4组间葡萄糖水平及其他生化指标差异均不明显。原始标本为有菌尿液时:尼泊金丁酯组菌量与原始标本组相比明显减少(P<0.05);甲苯组、无防腐剂组与原始标本组比较,差异无统计学意义(P>0.05);尼泊金丁酯组的葡萄糖水平与处理前的原始标本组比较,差异无统计学意义(P>0.05),而未加防腐剂组、甲苯组的葡萄糖水平与原始标本比较明显下降(P<0.05);尼泊金丁酯组、甲苯组、无防腐剂组的其余生化指标包括离子、肌酐、尿素、微量总蛋白,与原始标本组比较,差异均无统计学意义(P>0.05)。结论尼泊金丁酯作为防腐剂在24h尿液生化检查中,对离子、微量总蛋白、肌酐及尿素结果无明显影响。且对尿标本中的病原菌有较强的抑制能力,可防止葡萄糖的分解,维持该指标的稳定性。

Objective To study the effects of butylparaben used as a preservative on the biochemical determination of 24-hour urine specimen. Methods Fifteen fresh urine specimens of the out patients or in inpatients were collected.Each sample was divided into 4 parts.One part of the original specimen was used to examine the contents of microdose albumin （M.Pr）,glucose （GLU）,ions of potassium （K）,sodium Na,chlorine （Cl）,calcium（Ca）,and phosphorus （P）,urea nitrogen （UREA）,creatinine （CREA）,and bacteria number.The rest were divided into three groups as follows,toluene-treated group,butylparaben-treated group,without preservative-treated group.After 24 hours,all the groups were quantitatively detected the items of M.Pr,GLU,K,Na,Cl,Ca,P,UREA,CREA,and bacteria（or fungus） number. Results The biochemical indexes including ions （K,Na,Cl,Ca,P）,UREA,CREA,M.Pr,were both no difference between the four groups （ P 〉0.05）.There was no difference for the glucose between the butylparaben-treated group and the original specimen.However,the glucose level of the toluene-treated group or without preservative-treated group was significantly lower than that of the original group （ P 〈0.05）.The bacteria number of butylparaben group was significantly less than that of the original specimen,the toluene group and no preservative group （ P 〈0.05）. Conclusion Butylparaben as a powerful bacteriostatic agent,has a good effect on inhibition of glucose fermentation and no effect on the results of ions （K,Na,Cl,Ca,P）,M.Pr,UREA and CREA in 24-hour urine specimen.Our report suggests that butylparaben has the potential to be a new preservative used for 24-hour urine biochemical examination.