补益心脾对产后抑郁症大鼠模型Th1/Th2平衡细胞免疫调节作用的影响

Effects of Nourishing the Heart and Spleen on Th1/Th2 Balance Cellular Immune Regulation in Postpartum Depression Rats

目的:观察产后抑郁症大鼠模型Th1和Th2细胞比例的变化并探讨参芪解郁方对产后抑郁症Th1/Th2平衡的调节作用机制。方法:选取SD雌性大鼠90只,随机分为正常组、模型组、假手术组、中药组、西药组,每组6只,设灌胃1、2、4周3个时间点。采用流式细胞术检测3个时间点各组大鼠外周血Th1细胞和Th2细胞比例,并计算Th1/Th2比值。结果:1周模型组Th1及Th2细胞比例较正常组降低,差异有统计学意义(P <0. 05),2周、4周模型组较正常组升高,差异有统计学意义(P <0. 05); 1周模型组Th1/Th2比值较正常组升高,差异有统计学意义(P <0. 05),2周、4周模型组较正常组降低,差异有统计学意义(P <0. 05) 3、1周中药组Th1细胞比例较模型组升高,2周、4周中药组较模型组降低,差异有统计学意义(P <0. 05)。1周中药组Th2细胞比例较模型组升高(P <0. 05); 2周、4周中药组较模型组降低,差异有统计学意义(P <0. 05)。1周中药组Th1/Th2比值较模型组降低; 2周中药组较模型升高(P <0. 05),4周中药组升高明显,差异有统计学意义(P <0. 05)。结论:Th1/Th2平衡细胞参与产后抑郁症大鼠模型的发生发展,参芪解郁方通过对产后抑郁症Th1/Th2失衡状态的调节而发挥其效用。

Objective：To observe the change of the proportion of Thl cells to Th2 cells in postpartum depression （PPD） rats,and to explore the mechanism of Shenqi Jieyu Decoction （SJD） on regulating the balance of PPD Th1/Th2 from T cell immunity. Methods ： A total of 90 female SD rats were divided into normal group, model group, sham-operated group, SJD group and medicine group with 6 rats in each group. The rats were administrated for 1,2,and 4 weeks. The proportion of Th1 cells to Th2 cells in peripheral blood of each group was observed with flow cytometry in 3 times, and file ratio of Tbl/Th2 was calculated. Results：Proportion of Thl cells to Th2 cells in model group was lower than that in normal group （ P 〈 0. 01 ） ; and it increased in 2-week and 4- week time point than that in normal group （ P 〈 0. 01 ） ; Proportion of Th1 to Th2 in model group was increased in 1 -week time point than that in normal group （ P 〈 0. 05 ）, while it was lower in 2-week and 4-week time point （ P 〈 0. 01 ） ; Thl cells in SJD group were increased in 1 -week time point compared with that in model group （ P 〉 0. 05 ） , while it decreased in 2-week and 4- week time point （P 〈 0. 01 ）. The proportion of Th2 cells in SJD group increased in 1 -week time point compared with the model group （ P 〈 0. 05 ）, while it decreased in 2-week and 4-week time point （ P 〈 0. 01 ）. Proportion of Thl to Th2 was decreased in SJD group in 1 -week time point compared with that in model group （ P 〉 0. 05 ） ; It was increased in the 2-week time point （ P 〈 0. 05 ）, while it increased in 4-week time point （P 〈 0. 01 ）. Conclusion： Imbalance in the ratio between Th1 and Th2 may be a potent pathogenesis of PPD, and therapeutic effect of SJD on PPD is worked by regulating Thl cells and Th2 cells.