前列宁胶囊调控MMP-2影响细胞外基质对良性前列腺增生治疗的影响

Effects of Qianliening Capsules in Regulating Extracellular Matrix via MMP-2 on Treating Benign Prostatic Hyperplasia

目的:观察前列宁胶囊(QC)对基质金属蛋白酶-2(MMP-2)介导的细胞外基质(ECM)的影响,探讨QC对BPH的治疗机制。方法:SD大鼠随机分为空白组,BPH模型组,QC低、中、高观察组,建立BPH模型,QC干预,ELISA法检测大鼠血清MMP-2、FN、Collagen IV、LN; BPH-1细胞培养,分别加入MMP-2和未加MMP-2刺激因子,QC干预,MTT法观察细胞活性,Q-PCR及Western-Blot分别检测MMP-2、FN、Collagen IV、LN基因及蛋白表达(未加MMP-2),加入MMP-2组检测FN、Collagen IV、LN。结果:QC各剂量组大鼠血清中的FN、LN、Collagen IV含量降低,MMP-2升高(P <0. 05 or P <0. 01);BPH-1细胞培养QC干预,加入及未加入MMP-2刺激因子细胞活力均有下降,以48 h后最明显;加入MMP-2刺激因子FN、Collagen IV、LN基因及蛋白表达明显低于未加MMP-2刺激因子,差异有统计学意义。结论:QC对BPH具有治疗作用,QC调控MMP-2介导细胞外基质FN、Collagen IV、LN基因和蛋白表达可能是其治疗BPH机制之一。

Objective：To observe the effects of Qianliening Capsules （QC） on the extracellular matrix （ECM） via MMP-2 in treating benign prostatic hyperplasia （BPH） , and to explore the therapeutic mechanism of QC on BPH. Methods：Sprague-Dawley rats were randomly divided into blank group, BPH model group, QC low, middle, and high treatment groups. BPH rat model was estab- lished and intervened by QC. The MMP-2, LN, FN, Collagen IV in serum were examined by ELISA. BPH-1 cells without MMP-2 stimulators and BPH-1 cells added with MMP-2 stimulators were cultured respectively and treated by QC. The cell morphology and viability- were observed by light microscope and MTT. The mRNA and protein expression of MMP-2, LN, Collagen IV and FN were detected by Q-PCR and Western blot assays （no MMP-2）, LN, Collagen IV and FN were detected by Q-PCR and Western blot assays （MMP-2 was added）. Results：The levels of Collagen IV, FN, LN in serum of BPH rats serum treated with QC groups were re- duced significantly and MMP-2 was significantly increased （ P 〈 0. 05 or P 〈 0. 01 ）. The mRNA and protein expression of LN, FN, Collagen IV in BPH-1 cells QC-treated groups were decreased significantly, and the result was more obvious in the MMP-2 added groups, but the MMP-2 was enhanced in BPH-1 cells QC-treated groups, with significant difference. Conclusion：QC has significant therapeutic effects on BPH. QC can inhibit the expression of FN, Collagen IV and LN,and enhance MMP-2, and MMP-2 can regulate FN, Collagen IV and LN, QC regulate the expression of ECM via MMP-2 is one of the mechanisms by which QC treats BPH.