细胞学方法评价南美草药阿萨伊的寒热药性

Evaluation on Hot/Cold Characteristics of A9aí ( Euterpe oleracea Mart. ) Using Cytological Methods

目的:评价南美草药阿萨伊的寒热药性。方法:采用MTT细胞增殖抑制试验对比考察阿萨伊、黄柏对细胞株Hep G2和Hep3B增殖的影响;倒置相差显微镜对比观察二者对2种细胞株形态特征的影响;台盼蓝染液排斥试验对比分析二者的细胞毒性作用;分光光度法测定阿萨伊处理后的Hep G2和Hep3B细胞NADH含量和NADH/NAD+比值的变化,分析其对2种细胞能量代谢的影响。结果:在受试浓度范围内,阿萨伊、黄柏对2种细胞株的生长增殖和能量代谢均呈现不同程度的抑制作用。MTT试验中,阿萨伊浓度≤0. 5μg/m L和≥10μg/m L时对2种细胞产生增殖抑制效应,差异有统计学意义(P <0. 05; P <0. 01),且与黄柏作用相似;镜检下细胞的生长疏松程度与上述结果一致,阿萨伊使细胞颗粒感减弱,扁平呈不规则多边形铺展,与黄柏作用相似;台盼蓝染色结果显示各浓度阿萨伊对细胞无明显毒性,亦与黄柏作用相似;阿萨伊对2种细胞胞内能量代谢指标NADH含量、NADH/NAD+比值均有下调作用,差异有统计学意义(P <0. 05; P <0. 01)。结论:阿萨伊作用于细胞株Hep G2和Hep3B后与黄柏作用相似,能够降低能量代谢,其药性偏寒凉。

Objective：To evaluate the hot/cold characteristics of Acai. Methods：MTT cell proliferation inhibition assay was performed to study Acai＇s effect on cell proliferation. Inverted phase contrast microscopy was applied to study Acai＇s effect on cell morphological features. Trypan Blue staining exclusion assay was used to compare and analyze Acai＇s cytotoxicity. A further determination of cellular NADH level and NADH/NAD ＋ ratio via spectrophotometry was performed to analyze Acai＇s effect on cell energy metabolism. Results ：Within the range of preset concentrations, Acai and Cortex Phellodendri demonstrated divergently inhibitive effects on cell growth, proliferation and energy metabolism of the two hepatic cell strains. In the MTT assay, Acai showed significant （P 〈0. 05） or highly significant （P 〈 0. 01 ） inhibitive effect on cell proliferation at concentrations -〈0. 5 -g/mL and i〉 10 -g/ mL. Microscopic examination showed corresponding results of lower cell density, attenuated granularity, and more outstretched cells in irregular polygonal shape could be observed. Trypan Blue staining assay showed that any concentration of Acai had no obvious toxicity to the cells ,which was similar to Cortex Phellodendri. Acai with any preset concentrations was also found to be significantly （P 〈0. 05） or highly significantly （P 〈 0. 01 ） reducing intracellular NADH level as well as NADH/NAD ＋ ratio. Conclusion： The effects of Acai on cell lines HepG2 and Hep3 B are similar to that of Cortex Phellodendri Chinensis, and can reduce energy metabolism. This study proves that the medicinal property of Acai is cold or cool.