N5,N10-亚甲基四氢叶酸还原酶基因多态性与冠心病合并2型糖尿病的相关性研究

Analysia of the correlation between the polymorphism of N5,N10-methylenetetrahydrofolate reductase gene and coronary heart disease complicated with type 2 diabetes mellitus

目的探讨N5,N10-亚甲基四氢叶酸还原酶基因多态性与冠心病合并2型糖尿病的相关性。方法选取2017年1月～2018年9月我院收治的90例冠状动脉造影患者作为研究对象,其中正常者30例作为对照组,冠心病患者33例作为A组,冠心病合并2型糖尿病患者27例作为B组,均行N5,N10-亚甲基四氢叶酸还原酶基因多态性与生化指标(总胆固醇、三酰甘油、空腹血糖、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇)检测。结果 A组患者总胆固醇、三酰甘油、低密度脂蛋白胆固醇高于对照组(P<0.05);A组患者高密度脂蛋白胆固醇低于对照组(P<0.05);B组患者总胆固醇、三酰甘油、空腹血糖、低密度脂蛋白胆固醇高于A组和对照组(P<0.05);B组患者高密度脂蛋白胆固醇低于A组和对照组(P<0.05);对照组患者基因型为CC(95.0%)、CT(5.0%)、TT(0.0%),A组患者基因型为CC(68.8%)、CT(25.0%)、TT(6.2%),B组患者基因型为CC(27.8%)、CT(38.9%)、TT(33.3%),三组间比较,差异有统计学意义(P<0.05);对照组患者C等位基因频次>A组患者C等位基因频次>B组患者C等位基因频次(P<0.05)。结论 N5,N10-亚甲基四氢叶酸还原酶基因多态性与冠心病合并2型糖尿病的关系密切。TT基因型越多,冠心病合并2型糖尿病的患病风险越高。

Objective To investigate the correlation between the polymorphism of N5, N10-methylenetetrahydrofolatereductase gene and coronary heart disease （CHD） complicated with type 2 diabetes mellitus （DM）. Methods A total of90 patients performed with coronary angiography treated in our hospital from January 2017 to September in 2018 wereselected as the subjects. Among them, 30 patients were selected as normal control group. The 33 patients with CHDalone were classified into group A, and the rest 27 patients with CHD and type 2 DM were categorized into group B.They were all tested with N5, N10-methylenetetrahydrofolate reductase gene polymorphisms and biochemical markerssuch as total cholesterol （TC）, triacylglycerol, fasting blood glucose （FBG）, high-density lipoprotein cholesterol （HDL-C）, and low-density lipoprotein cholesterol （LDL-C）. Results The levels of TC, triacylglycerol, and LDL-C in group Awere higher than those in the control group （P〈0.05）. The HDL-C level in the group A was lower than that in the control group （P〈0.05）. The TC, triglyceride, FBG and LDL-C in the group B were higher than those in the group A andcontrol group （P〈0.05）. The HDL-C level in the group B was lower than that in the group A and the control group （P〈0.05）. The genotypes of patients in the control group were CC （95.0%）, CT （5.0%） and TT （0.0%）. In the group A, the genotypes were CC （68.8%）, CT （25.0%） and TT （6.2%）. In the group B, the patients＇ genotypes were CC （27.8%）, CT（38.9%）, TT （33.3%）. After inter-group comparisons, there were statistical significance （P〈0.05）. The sequence of frequency of C alleles was listed as control group, group A, and group B from high to low （P〈0.05）. Conclusion The N5,N10-methylenetetrahydrofolate reductase gene polymorphism is closely associated with CHD complicated with type 2DM.The more TT genotypes are,the higher the risk of CHD with type 2 DM becomes.