石榴皮多糖的提取、纯化和结构分析

Extraction,Purification,and Structure Analysis of Polysaccharides from Punica granatum Peel

选用石榴皮为原料,在单因素实验的基础上采用正交实验优化了石榴皮多糖的提取条件;采用三氯乙酸法和大孔吸附树脂脱除石榴皮粗多糖中的蛋白质和色素;采用全自动蛋白多糖纯化系统对纯化后的石榴皮多糖进行分离,并利用红外光谱对分离的组分进行结构分析。结果表明:石榴皮多糖的最佳提取条件为乙醇体积分数20%、提取温度80℃、提取时间6h、料液比1∶20(g∶mL),在此条件下,石榴皮多糖提取率达到9.45%;三氯乙酸法脱蛋白率为88%;5种大孔吸附树脂中S-8型大孔吸附树脂的除色素效果最佳,色素去除率为75%;利用全自动蛋白多糖纯化系统分离出SLP-1、SLP-2、SLP-3三种组分;红外光谱分析表明,SLP-1组分具有甘露糖的特征吸收峰,三种组分均含有吡喃环结构。

Using Punica granatum peel as a raw material,we optimized the extraction conditions of polysaccharides fromPunica granatum peel by orthogonal experiments on the basis of single-factor experiments,and removed protein and pigment from crude polysaccharides from Punica granatum peel by a trichloroacetic acid method and macroporous adsorption resin.Moreover,we separated the purified polysaccharides from Punica granatum peel by an automatic proteoglycan purification system,and analyzed the structure of the separated components by infrared spectroscopy.The results show that the optimum extraction conditions of polysaccharides fromPunica granatumpeel are as follows：the ethanol volume fraction of 20%,the extraction temperature of 80℃,the extraction time of 6 h,and solid-liquid ratio of 1∶20（g∶mL）.Under above conditions,the extrac-tion rate of polysaccharides fromPunica granatum peel reaches 9.45%.The removal rate of protein by the trichloroacetic acid method is 88%.Among the five macroporous adsorbent resins,S-8 has the best effect on pigment removal,and the removal rate is 75%.In addition,three components of SLP-1,SLP-2,and SLP-3 are isolated by the automatic proteoglycan purification system.Infrared spectrum analysis shows that the SLP-1 component contains the characteristic absorption peak of mannose,and all the three components contain a pyran ring structure.