摘要
本研究以70%乙醇溶液浸提干燥的大青叶,用分光光度法测定提取液中的粗黄酮含量。在单因素试验的基础上,通过正交试验优化了大青叶粗黄酮的提取工艺。最佳提取条件为:提取时间为120min、提取温度为80℃、料液比为1:25,粗黄酮提取率最高为3.62%。抑菌试验的结果显示大青叶粗黄酮对大肠杆菌、金黄色葡萄球菌和李斯特菌有明显抑制作用。本研究为大青叶的进一步开发利用提供了理论基础。
In this study,crude flavonoids was extracted from Folium Isatidis with 70% ethanol and its content was determined by speetrophotometry-. On the basis of single factor experiment, the extraction process of crude flavonoids from Folium Isatidis was optimized by orthogonal test. The optinmnl extraction conditions were as follows： the extraction time was 120min, the extraction temperature was 80 ℃, the solid - liquid ratio was 1 ： 25. The extraction rate o fflavonoids was 3.62%. The antibacterial activity of the extract was also investigated. The inhibitory- effects on Eseheriehia eoli, Staphylococcus aureus and Listeria monoeytogenes were significant, which laid a theoretical foundation for the further development and utilization of Folium Isatidis.
作者
刘富康
方士元
曲映红
Liu Fukang;Fang Shiyuan;Qu Yinghong(Shanghai Ocean University,National Experimental Teaching Demonstration Center for Food Science and Engineering,Shanghai 201306,China)
出处
《山东化工》
CAS
2018年第21期34-35,38,共3页
Shandong Chemical Industry
关键词
大青叶
黄酮
提取工艺
抑菌性
Folium Isatidis
flavonoids
extraction technology
antibacterial activity
