UPLC法测定食品中10种合成着色剂的含量

Determination of 10 synthetic colorants in food by UPLC

采用UPLC法建立了测定食品中10种合成着色剂,优化了不同食品前处理方法,聚酰胺小柱净化后,进行UPLC分析定量。结果显示:10种合成色素线性关系R2≥0.9996,具有较好的线性关系;且检测限为0.0005 g/kg～0.0041 g/kg,加标回收率在62.13%～101.15%,变异系数RSD在0.59%～1.89%之间。该方法能够快速测定食品中10种合成着色剂,具有准确、灵敏和稳定的特点。

The UPLC method was used to establish 10 synthetic colorants in food products,optimize the pretreatment methods of different foods,and used polyamide column to purify and analyze UPLC.Results showed that：the linear relationship was good with determination coefficients R2≥0.9996 in the 10 synthetic colorants.The recoveries of standard addition ranged from 62.13% to 101.15 %,the limit of quantification was between 0.0005 g/kg to 0.0041 g/kg,and coefficient of variation（RSD）of 0.59%~1.89 %.The method can quickly determine 10 synthetic colorants in food,which is accurate,sensitive and stable.