HPLC法测定1-氟萘中亚硝酸钠杂质含量

Determination of Sodium nitrite in 1-Fluoronaphthalene by HPLC

目的:建立1-氟萘中亚硝酸钠杂质的含量检测的HPLC方法。方法:采用Agilent 1200色谱系统;色谱柱为Zorbax SB-AQ,250 mm×4.6 mm,5μm;流动相为0.03 mol/L磷酸二氢钾缓冲液,用磷酸调节pH为3.5,等度洗脱;流速0.7 mL/min;柱温30℃;检测波长为210 nm;进样量为20μL。结果:亚硝酸钠可与其副产物硝酸钠完全分离,检测限为0.1 mg/kg。结论:该方法专属性好,灵敏度高、且操作简便,可用于1-氟萘原料中亚硝酸钠杂质的控制。

：Objective：A HPLC method for determination of sodium nitrite in 1-Fluoronaphthalene was established. Method：Use Agilent1200 chromatography,Zorbax SB-AQ,250 mm×4.6 mm,5 μm as column,nitrogen as the carrier gas,0.03M monopotassium phosphate buffer solution,adjust pH to 3.5 with orthophosphoric acd as mobile phase,chose isocartic elution mode;the flowrate is 0.7 ml/min,the column temperature is 30℃,the detection wavelength is 220 nm and the injection volume is 20 μL.Results：The results show sodium nitrite can be separated well from its by product sodium nitrate,the limit of detection is 0.1 mg/kg.Conclusion：This method is specificity,sensitive and simple and can be used for determindation of sodium nitrite in 1-Fluoronaphthalene.