EV71感染手足口病严重程度与IL-6、IL-1β基因多态性的相关性研究

Research on the association of interleukin-6 and interleukin-1β gene polymorphism with severity of hand foot and mouth disease induced by enterovirus 71

目的探讨炎性细胞因子白介素-6(IL-6)、IL-6R、IL-1β、IL-1RN基因多态性与肠道病毒71型(EV71)感染手足口病(HFMD)的严重程度的相关性,为重症HFMD的早期发现和患病风险的评估提供依据。方法以西安市儿童医院和西安交通大学第二附属医院感染科收住的180例EV71HFMD患儿为研究对象,其中重症组108例,普通组72例。采用SNPscanTM多重SNP技术检测上述样本IL-6rs1800796、rs2069837,IL-6Rrs2228145、rs4845617,IL-1βrs16944、rs1143627,IL-1RN rs315952、rs9005位点的基因分型。结果 180例EV71感染患儿中,IL-6rs1800796G等位基因的重症化风险升高(OR=1.312,95%CI:1.041～1.653),IL6-R rs2228145C等位基因重症化风险升高(OR=1.330,95%CI:1.064～1.663),而IL-1βrs1143627G等位基因是保护因素(OR=0.790,95%CI:0.635～0.981)。IL-6rs2069837、IL-6R rs4845617,IL-1βrs16944、IL-1RN rs315952、rs9005的基因多态性与疾病严重程度无明显相关性。结论 IL-6rs1800796、IL-6Rrs2228145、IL-1βrs1143627基因多态性与EV71HFMD患儿严重程度相关联。

Objective To investigate the association of inflammatory cytokines including interleukin-6（IL-6）,IL-6 R,IL-1βand IL-1 RN gene polymorphism with severity of hand foot and mouth disease（HFMD）induced by enterovirus 71（EV71）,in order to provide evidence for early recognition and assessment of severe HFMD. Methods A total of 180 EV71 HFMD cases including 108 severe cases and 72 mild cases from the Second Affiliated Hospital of Xi′an Jiaotong University and Xi′an Children′s Hospital were randomly enrolled in this study.All participants were genotyped for the most common IL-6,IL-6 R,IL-1βand IL-1 RN single-nucleotide polymorphisms（SNPs）using the SNPscan multiple SNP typing method.The gene type of rs1800796,rs2069837 of IL-6,rs2228145,rs4845617 of IL-6 R,rs16944,rs1143627 of IL-1βand rs315952,rs9005 of IL-1 Ramong 180 cases were detected by SNPscan kit. Results The severity risk was significantly increased in IL-6 rs1800796 allele G（OR=1.312,95%CI：1.041～1.653）and IL6-R rs2228145 allele C（OR=1.330,95%CI：1.064～1.663）,while it was reduced in IL-1βrs1143627 allele G（OR=0.790,95%CI：0.635～0.981）.No association of gene polymorphism with severity of EV71 HFMD was observed in IL-6 rs2069837,IL-6 Rrs4845617,IL-1βrs16944,IL-1 RN rs315952 and IL-1 RN rs9005. Conclusion The gene polymorphism of IL-6 rs1800796,IL-6 Rrs2228145 and IL-1βrs1143627 were associated with severity of EV71 HFMD.